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OwnerStuart Alan Kauffman, Marc Ballivetinventors
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The present invention is directed to a process for the production of a peptide, polypeptide, or protein having a predetermined property. In accordance with one embodiment, the process begins by producing by way of synthetic polynucleotide coupling, stochastically generated polynucleotide sequences. A library of expression vectors containing such stochastically generated polynucleotide sequences is formed. Next, host cells containing the vectors are cultured so as to produce peptides, polypeptides, or proteins encoded by the stochastically generated polynucleotide sequences. Screening or selection is carried out on such host cells to identify a peptide, polypeptide, or protein produced by the host cells which has the predetermined property. The stochastically generated polynucleotide sequence which encodes the identified peptide, polypeptide, or protein is then isolated and used to produce the peptide, polypeptide, or protein having the predetermined property.
A method of identifying a peptide, polypeptide or protein having a binding property to a ligand, comprising:
An isolated, diverse population of peptides, polypeptides, or proteins comprising greater than about 1.times.10.sup.5 different stochastic amino acid sequences encoded by stochastic polynucleotide sequences.
A method of isolating a polynucleotide sequence encoding a peptide, polypeptide or protein having a predetermined binding property to a ligand, comprising:
An isolated, diverse population of polynucleotide sequences which encode a diverse population of ligand binding peptides, polypeptides, or proteins comprising greater than about 1.times.10.sup.5 different stochastic amino acid sequences encoded by the stochastic polynucleotide sequences.
A method of isolating a peptide, polypeptide or protein having a binding property, comprising:
A method of producing a diverse population of stochastically generated polynucleotide sequences encoding a diverse population of ligand binding peptides, polypeptides or proteins, comprising:
An isolated, diverse population of vectors comprising stochastically generated polynucleotide sequences encoding a diverse population of ligand binding peptides, polypeptides, or proteins comprising greater than about 1.times.10.sup.5 different stochastic amino acid sequences.
An isolated, diverse population of peptides or polypeptides comprising stochastic amino acid sequences encoded by stochastic polynucleotide sequences of about 300 nucleotides or less in length.
An isolated, diverse population of polynucleotides sequences encoding a diverse population of ligand binding peptides or polypeptides comprising stochastic amino acid sequences encoded by stochastic polynucleotide sequences of about 300 nucleotides or less in length.
An isolated, diverse population of vectors encoding a diverse population of ligand binding peptides or polypeptides comprising stochastic amino acid sequences encoded by stochastically generated polynucleotide sequences of about 300 nucleotides or less in length.
An isolated, diverse population of peptides, polypeptides, or proteins comprising stochastic amino acid sequences produced by a method comprising synthesizing a diverse population of stochastically generated polynucleotide sequences selected from a method consisting of stochastic copolymerization of double stranded oligonucleotides, copolymerization of the four kinds of nucleotide triphosphates consisting of Adenine, Cytosine, Guanine and Thymine, and chemical synthesis;
Dependencies are inferred from the claim text.