Culture medium for rapid count of coliform bacteria
AssigneeMinnesota Mining and Manufacturing Company
InventorsPatrick A. Mach, Karen E. Hesselroth, Carl A. Adams, Debra L. Schwab
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Abstract
This invention generally relates to products and processes used to determine the presence of bacteria in a sample and particularly relates to a culture medium which may be used in products and processes to allow early detection and count of Enterobacteriaceae. The bacterial culture medium which facilitates the early detection and count of bacteria is a mixture of gelatin peptone and yeast extract, lactose or glucose, sodium chloride, bile salts, guar gum and an excess amount of a sulfonphthalein dye sufficient to provide a high concentration of dye in close proximity to the growing bacteria in order to allow detection and count of the growing bacteria.
Claims26
A bacterial culture medium which facilitates the early detection and count of Enterobacteriaceae growing in the medium comprising tryptose or a mixture of gelatin peptone and yeast extract, lactose or glucose, sodium chloride, bile salts, guar gum and an excess amount of a sulfonphthalein dye sufficient to provide a high concentration of dye in close proximity to the growing bacteria in order to allow detection and count of the growing bacteria.
A method of detecting the presence of Enterobacteriaceae in a sample comprising the steps of adding an aliquot of the sample containing bacteria to a culture medium comprising a mixture of gelatin peptone and yeast extract, lactose or glucose, sodium chloride, bile salts, guar gum and an excess amount of a dye sufficient to provide a high concentration of dye in close proximity to the bacteria;
- growing the bacteria in the presence of the culture medium;
- and detecting the color change of the dye as the growing bacteria metabolize.
A device to detect coliform bacterial growth in a sample consisting essentially of a self-supporting, waterproof substrate, a foam spacer and a transparent cover sheet, wherein a culture medium comprising a mixture of gelatin peptone and yeast extract, lactose or glucose, sodium chloride, bile salts, guar gum and an excess amount of a sulfonphthalein dye is coated on the self-supporting, waterproof substrate sufficient to provide a high concentration of dye in close proximity of growing bacteria in order to allow detection and count of the growing bacteria.
Dependencies are inferred from the claim text.