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US 5,723,286 AGranted
Peptide library and screening systems
AssigneeAffymax Technologies N.V.
InventorsWilliam J. Dower, Steven E. Cwirla, Ronald W. Barrett
Life of this patent
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Abstract
Peptides which bind to selected receptor molecules are identified by screening libraries which encode a random or controlled collection of amino acids. Peptides encoded by the libraries are expressed as fusion proteins of bacteriophage coat proteins, and bacteriophage particles are then screened against the receptors of interest. Peptides having a wide variety of uses, such as therapeutic or diagnostic reagents, may thus be identified without any prior information on the structure of the expected ligand or receptor molecule.
Claims45
A method for identifying a peptide which binds to a preselected receptor molecule, comprising:
- transforming host cells by electroporation with at least 10.sup.8 different bacteriophage expression vectors, wherein each of said different vectors encodes a fusion protein comprising a peptide fused to a coat protein of a filamentous bacteriophage so that the N-terminal amino acid of said fusion protein is the N-terminal amino acid of said peptide, and wherein said different vectors are constructed by ligating each oligonucleotide of a mixture of at least 10.sup.8 different oligonucleotides to a bacteriophage cloning vector that encodes the coat protein so as to form a coding sequence for said fusion protein, wherein each of said different oligonucleotides comprises a series of codons encoding a random collection of amino acids and encodes a different peptide;
- cultivating the transformed cell under conditions suitable for expression and assembly of bacteriophage particles thereby displaying said peptides on the surface of said particles;
- and
- selecting bacteriophage particles displaying the peptide by combining said particles with the preselected receptor molecule and separating particles bound to said receptor molecule from unbound particles.
1 more element
A method for identifying a peptide which binds to a preselected receptor molecule, comprising:
- transforming host cells by electroporation with at least 10.sup.8 different bacteriophage expression vectors, wherein each of said different vectors encodes a fusion protein comprising a peptide fused to a coat protein of a filamentous bacteriophage, and wherein said different vectors differ from each other with respect to the peptide of said fusion protein encoded by said vector;
- cultivating the transformed cell under conditions suitable for expression and assembly of bacteriophage particles thereby displaying said peptides on the surface of said particles;
- and
- selecting bacteriophage particles displaying the peptide by combining said particles with the preselected receptor molecule and separating particles bound to said receptor molecule from unbound particles
- wherein each of said 10.sup.8 different oligonucleotides is composed of first, second, and third single-stranded oligonucleotides that are annealed to one another prior to insertion into said cloning vector;
- and
- wherein said first single stranded oligonucleotide is .sup.5' C T C T C A C T C C (NNK).sub.x A C T G T T G A A A G T T G T;
- and said second and third single stranded oligonucleotides are .sup.5' G G A G T G A G A G T A G A and .sup.5' C T T T C A A C A G T, respectively.
5 more elements
A method for identifying a peptide which binds to a preselected receptor molecule, comprising:
- transforming host cells by electroporation with at least 10.sup.8 different bacteriophage expression vectors, wherein each of said different vectors encodes a fusion protein comprising a peptide fused to a coat protein of a filamentous bacteriophage, and wherein said different vectors differ from each other with respect to the peptide of said fusion protein encoded by said vector;
- cultivating the transformed cell under conditions suitable for expression and assembly of bacteriophage particles thereby displaying said peptides on the surface of said particles;
- and
- selecting bacteriophage particles displaying the peptide by combining said particles with the preselected receptor molecule and separating particles bound to immobilized preselected receptor molecule from unbound particles;
- repeating the selection step at least once, wherein the selected bacteriophage particles are propagated between said selection steps
- wherein said receptor is immobilized at reduced densities in subsequent repetitions of the selecting step.
3 more elements
A method for identifying a peptide which binds to a preselected receptor molecule, comprising:
- transforming host cells by electroporation with at least 10.sup.8 different bacteriophage expression vectors, wherein each of said different vectors encodes a fusion protein comprising a peptide fused to a coat protein of a filamentous bacteriophage, and wherein said different vectors differ from each other with respect to the peptide of said fusion protein encoded by said vector;
- cultivating the transformed cell under conditions suitable for expression and assembly of bacteriophage particles thereby displaying said peptides on the surface of said particles;
- and
- selecting bacteriophage particles displaying the peptide by combining said particles with the preselected receptor molecule and separating particles bound to said receptor molecule from unbound particles, wherein said bacteriophage displaying the peptide are combined with said preselected receptor molecule under conditions favoring monovalent binding.
1 more element
Dependencies are inferred from the claim text.