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AssigneeDepoTech Corporation
InventorsSinil Kim, Stephen B. Howell
Estimating the term…
Disclosed are multivesicular liposomes containing biologically active substances, the multivesicular liposomes having defined size distribution, adjustable average size, adjustable internal chamber size and number, and a modulated rate of the biologically active substance in contrast to the previous art. The process comprises dissolving a lipid component in volatile organic solvents, adding an immiscible aqueous component containing at least one biologically active substance to be encapsulated, and adding to either or both the organic solvents and the lipid component, a hydrochloride effective to control the release rate of the biologically active substance from the multivesicular liposome, making a water-in-oil emulsion from the two components, immersing the emulsion into a second aqueous component, dividing the emulsion into small solvent spherules which contain even smaller aqueous chambers, and then removing the solvents to give an aqueous suspension of multivesicular liposomes encapsulating biologically active substances.
A multivesicular liposome having multiple non-concentric chambers with membranes distributed in a matrix comprising:
A method for the treatment of a patient in need thereof with a biologically active substance selected from the group consisting of an anesthetic, an antiasthmatic agent, a cardiac glycoside, an antihypertensive, a nucleic acid, an antibiotic, a vaccine, an antiarrhythmic, an antiangina, a hormone, an antidiabetic, an antineoplastic, an immunomodulator, an antifungal, a tranquilizers, a steroid, a sedative, an analgesic, a vasopressor, an antiviral, a herbicide, a pesticide, a protein, a peptide, a neurotransmitter, a radionuclide, and suitable combinations thereof comprising administering said substance to the patient encapsulated in a multivesicular liposome wherein the encapsulation was done in the presence of a hydrochloride selected from the group consisting of hydrochloric acid, arginine hydrochloride, histidine hydrochloride, lysine hydrochloride, pyridine hydrochloride, and suitable combinations thereof, in a concentration in the range from about 10 mM to about 500 mM;
A multivesicular liposome composition comprising:
A method for the treatment of a patient in need thereof with a compound selected from the group consisting of cytarabine, morphine sulfate, hydromorphone, amikacin, and methotrexate, comprising administering said compound to the patient encapsulated in a multivesicular liposome wherein the encapsulation was done in the presence of a hydrochloride selected from the group consisting of hydrochloric acid, arginine hydrochloride, histidine hydrochloride, lysine hydrochloride and pyridine hydrochloride in a concentration in the range from about 10 mM to about 500 mM;
Dependencies are inferred from the claim text.