GnRH-leukotoxin chimeras
AssigneeUniversity of Saskatchewan
InventorsAndrew A. Potter, John G. Manns
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Abstract
New immunological carrier systems, DNA encoding the same, and the use of these systems, are disclosed. The carrier systems include chimeric proteins which comprise a leukotoxin polypeptide fused to a selected GnRH multimer which consists essentially of at least one repeating GnRH decapeptide sequence, or at least one repeating unit of a sequence corresponding to at least one epitope of a selected GnRH molecule. Under the invention, the selected GnRH sequences may all be the same, or may correspond to different derivatives, analogues, variants or epitopes of GnRH so long as the GnRH sequences are capable of eliciting an immune response. The leukotoxin functions to increase the immunogenicity of the GnRH multimer fused thereto.
Claims26
A chimeric protein comprising a leukotoxin polypeptide fused to a multimer having more than one selected GnRH polypeptide, whereby said leukotoxin portion of said chimeric protein acts to increase the immunogenicity of said GnRH multimer.
A chimeric protein comprising a leukotoxin polypeptide fused to a multimer having eight selected GnRH polypeptides, wherein the C-terminus of the leukotoxin polypeptide is fused to the N-terminus of the multimer.
A chimeric protein comprising a leukotoxin polypeptide fused to a multimer having eight selected GnRH polypeptides, wherein the C-terminus of the multimer is fused to the N-terminus of the leukotoxin polypeptide.
Dependencies are inferred from the claim text.