Apolipoprotein(A) promoter and regulatory sequence constructs and methods of use
AssigneeSandford University
InventorRichard Mark Lawn
Life of this patent
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Abstract
An apo(a) gene 5'-regulatory region upstream from position -208 from the translation initiation site is provided. Also included are expression constructs, vectors, translated cells, and transgenic animals comprising the regulatory region, and methods for determining expression levels and for screening for compounds which control expression.
Claims36
A nucleotide sequence comprising the human apolipoprotein(a) gene 5'-regulatory region from nucleotide position -208 to nucleotide position -1442 of FIG. 3 nucleotides 1-1235 of SEQ ID NO:
- 1), operatively linked to a heterologous sequence.
A nucleotide sequence comprising a human apolipoprotein(a) gene 5'-regulatory region operatively linked to a heterologous sequence, said 5'-regulatory region comprising at least one hundred consecutive nucleotides of the regulatory region depicted in FIG. 3 from nucleotide position -208 to -1442 nucleotides 1-1235 of SEQ ID NO:
- 1).
A vector comprising the human apolipoprotein(a) gene 5'-regulatory region from nucleotide position -208 to -1442 of FIG. 3 nucleotides 1-1235 of SEQ ID NO:
- 1), operatively linked to a heterologous sequence.
An isolated nucleotide sequence comprising at least thirty consecutive nucleotides (1) from the human apolipoprotein(a) gene 5'-regulatory region depicted in FIG. 3 from nucleotide position -208 to -1442 nucleotides 1-1235 of SEQ ID NO:
- 1) or (2) from the complement of said 5'-regulatory region.
A nucleotide sequence comprising at least fifteen nucleotides capable of forming a triplex DNA complex with a double stranded DNA comprising the human apolipoprotein(a) gene 5'-regulatory region depicted in FIG. 3 from nucleotide position -90 to -1442 nucleotides 1-1353 of SEQ ID NO:
- 1).
A vector comprising a human apolipoprotein (a) gene 5'-regulatory region operatively linked to a heterologous sequence, said 5'-regulatory region comprising at least one hundred consecutive nucleotides of the regulatory region depicted in FIG. 3 from nucleotide position -208 to -1442 nucleotides 1-1235 of SEQ ID NO:
- 1).
Dependencies are inferred from the claim text.