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US 5,721,120 AGranted
Method of disrupting cultured cells using an impinging jet device
AssigneeMerck & Co., Inc.
InventorsDouglas B. Seifert, Frank S. Leu
Life of this patent
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Abstract
A novel method of disrupting cells which do not have a cell wall comprises passing suspended cells through a low pressure impinging jet device. This method disrupts the cells, but does not harm the cell products which are liberated.
Claims10
A method for the disruption of cultured cells which lack a cell wall comprising passing cells suspended in a culture fluid through a low pressure impinging jet device.
A method of harvesting a cell product contained within a cell which does not have a cell wall comprising:
- a) culturing cells in a culture medium under culture conditions suited to bring about the production of the desired cell product;
- b) suspending the cells in suspension fluid;
- c) passing the suspended cells through a low pressure impinging jet device so that the cells are disrupted at a pressure of from about 5 to 100 psi and the cell product is released;
- and
- d) recovering the released cell product.
2 more elements
A method of harvesting a virus grown in an animal cell comprising:
- a) culturing animal cells infected with the virus;
- b) suspending the animal cells containing the virus in a suspension fluid;
- c) passing the suspended animal cells through a low pressure impinging jet device so that cells are disrupted and the virus is released;
- and
- d) harvesting the released virus.
2 more elements
Dependencies are inferred from the claim text.