Mammalian artificial chromosomes and methods of using same
AssigneeThe Regents of the University of California, San Diego
InventorImmo E. Scheffler
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Abstract
The present invention provides a mammalian artificial chromosome (MAC), comprising a centromere and a unique cloning site, said MAC containing less than 0.1% of the DNA present in a normal haploid genome of the mammalian cell from which the centromere was obtained. The invention further provides a MAC, wherein the unique cloning site is a nucleic acid sequence encoding a selectable marker. The invention also provides methods of preparing a MAC. In addition, the invention provides methods of stably expressing a selectable marker in a cell, comprising introducing a MAC containing the selectable marker into the cell. The invention also provides a cell containing a MAC expressing an exogenous nucleic acid sequence and a transgenic mammal expressing a selectable marker.
Claims17
A mammalian artificial chromosome (MAC) consisting of MAC-8.2.3 contained in host cell line XEW8.2.3, wherein said cell line has been deposited as Accession No. ATCC CRL 11992.
A MAC comprising MAC-8.2.3 and a unique cloning site, wherein the MAC is less than about 0.1% of the size of the normal haploid genome of the mammalian cell from which the centromere was obtained.
The mammalian cell of claim 31, wherein said cell is a human cell.
A method of preparing a MAC from MAC 8.2.3, comprising the steps of:
- (a) fragmenting MAC 8.2.3, and
- (b) selecting a centromeric fragment of said MAC 8.2.3, wherein said centromeric fragment contains less than about 0.1% of the DNA present in a normal haploid cell from which said MAC 8.2.3 was obtained.
The method of stably expressing a selectable marker in a mammalian cell, comprising introducing MAC 8.2.3 containing said selectable marker into said cell and stably expressing said selectable marker in said cell.
The method of producing an exogenous mammalian gene product in a mammalian cell, comprising the steps of:
- (a) introducing MAC 8.2.3 containing an exogenous nucleic acid sequence encoding the mammalian gene product into said mammalian cell, and
- (b) expressing said mammalian gene product in said mammalian cell.
Dependencies are inferred from the claim text.