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US 5,716,832 AGranted
Packaging cells
AssigneeChiron Viagene, Inc.
InventorsJack R. Barber, Douglas J. Jolly, James G. Respess, Stephen M. W. Chang
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AssigneeChiron Viagene, Inc.
InventorsJack R. Barber, Douglas J. Jolly, James G. Respess, Stephen M. W. Chang
Estimating the term…
The invention described herein allows the production of recombinant retroviruses (retroviral vector particles) from producer cells which are safer and of higher titre than normal. In addition, methods are provided for making helper cells which, when a recombinant retrovirus genome is introduced to make a producer line, produce particles that are targeted toward particular cell types. Methods are also provided for making recombinant retrovirus systems adapted to infect a particular cell type, such as a tumor, by binding the retrovirus or recombinant retrovirus in the particular cell type. Methods are also provided for producing recombinant retroviruses which integrate in a specific small number of places in the host genome, and for producing recombinant retroviruses from transgenic animals.
A method of producing a recombinant retrovirus, comprising growing a producer cell having a genome comprising:
A xenotropic packaging cell line which, upon introduction of a vector construct, produces viral particles uncontaminated by replication competent virus.
A polytropic packaging cell line which, upon introduction of a vector construct, produces vital particles uncontaminated by replication competent virus.
A polytropic packaging cell line wherein the packaging cell line, upon introduction of a vector construct, produces viral particles which infect cells of kidney lineage, but not cells or fibreblast, epithelial, T-cell or monocyte lineage.
A non-mouse packaging cell the carrying on separate operons the genes for gag/pol and env, said operons lacking retroviral LTR sequences and which, upon introduction of an N2 type vector construct, produces no helper virus after at least twenty days passage in culture.
A method of producing a recombinant retrovirus, comprising:
Dependencies are inferred from the claim text.