Clones encoding mammalian ADP-ribosylarginine hydrolases

An isolated nucleic acid consisting of a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:9 and SEQ ID NO:11, operably linked to a promoter.

A method of producing a mammalian ADP-ribosylarginine hydrolase by introducing into a suitable host cell an expression cassette having a promoter sequence operably linked to a DNA sequence consisting of sequences selected from the group consisting of Seq. I.D. No. 1 and Seq. I.D. No. 9 or to a human sequence encoding only a hydrolase wherein approximately 90% of the sequence is Seq. I.D. No. 11, and expressing said DNA sequence to produce said mammalian ADP-ribosylarginine hydrolase.

A method of isolating from mammalian tissue a DNA sequence encoding ADP-ribosylarginine hydrolase comprising, probing a DNA library prepared from mammalian tissue with an oligonucleotide probe which binds to a conserved portion of the nucleic acid of the rat brain ADP-ribosylarginine hydrolase cDNA within bases 418 to 912 of Seq. I.D. No. 1, wherein said probe is able to detect cDNA encoding ADP-ribosylarginine hydrolase with less than 1% false positives during the probing of the library, and separating any DNA sequence which binds to said probe.