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US 5,712,146 AGranted
Recombinant combinatorial genetic library for the production of novel polyketides
AssigneeLeland Stanford Junior University
InventorsChaitan Khosla, David A. Hopwood, Suzanne Ebert-Khosla, Robert McDaniel, Hong Fu, Camilla Kao
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Abstract
Novel polyketides and novel methods of efficiently producing both new and known polyketides, using recombinant technology, are disclosed. In particular, a novel host-vector system is described which is used to produce polyketide synthases which in turn catalyze the production of a variety of polyketides.
Claims5
A library for synthesis of a multiplicity of polyketides which library comprises
- a multiplicity of individual cell lines wherein each cell line produces a functional polyketide synthase (PKS) such that each said cell line produces a different polyketide;
- wherein each functional PKS comprises the products of:
- a first open reading frame (ORF) encoding a ketosynthase/acyltransferase (KS/AT);
- a second ORF encoding an acyl carrier protein (ACP);
- and a third ORF encoding a chain length determining factor (CLDF);
- wherein the ORFs in said library are derived from at least three different aromatic PKS.
3 more elements
Dependencies are inferred from the claim text.