Carboxy terminal peptide-extended proteins
AssigneeWashington University
InventorsIrving Boime, Martin M. Matzuk, Jeffrey L. Keene
Life of this patent
Estimating the term…
Abstract
Modified protein pharmaceuticals extended with a CTP unit retain their biological activity and they had extended biological half-lives. The CTP extension represents an amino acid sequence derived from that of positions 112-118 to 145 of human chorionic gonadotropin.
Claims9
A modified protein which retains the biological activity of the parent protein, wherein said modified protein comprises an extension of the carboxy terminus of said parent protein with an amino acid sequence of the carboxy terminal portion of human CG beta subunit (CTP) which consists of the amino acid sequence from amino acid 112-118 to position 145 of human CG, or a variant thereof wherein said variant differs from said CTP by 1-5 conservative amino acid substitutions.
A nucleic acid molecule which comprises a nucleotide sequence encoding a modified protein which retains the biological activity of the parent protein, wherein said modified protein comprises an extension of the carboxy terminus of said parent protein with an amino acid sequence of the carboxy terminal portion of human CG beta subunit (CTP) which consists of the amino acid sequence from amino acid 112-118 to position 145 of human CG, or a variant thereof wherein said variant differs from said CTP by 1-5 conservative amino acid substitutions.
A nucleic acid molecule which comprises an expression system effective to produce a modified protein which retains the biological activity of the parent protein, wherein said modified protein comprises an extension of the carboxy terminus of said parent protein with an amino acid sequence of the carboxy terminal portion of human CG beta subunit (CTP) which consists of the amino acid sequence from amino acid 112-118 to position 145 of human CG, or a variant thereof wherein said variant differs from said CTP by 1-5 conservative amino acid substitutions, which expression system comprises a nucleotide sequence encoding said modified protein operably linked to control sequences operable in a host cell.
Dependencies are inferred from the claim text.