Signal sequences for secretion of heterologous proteins from yeast
AssigneeKorea Institute of Science and Technology
InventorsBong Hyun Chung, Soo Wan Nam, Byung Moon Kim, Sun Ah Yang, Young Hoon Park
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Abstract
Secretion signal peptides of inulinases derived from Kluyveromyces marxianus, which makes a heterologous protein produced in a yeast cell be secreted almost completely into the extracellular medium; nucleotide sequences encoding said secretion signal peptides; expression and secretion vectors comprising nucleotide sequences encoding said secretion signal peptides; recombinant yeast cells transformed with said expression and secretion vectors; and a process for producing heterologous proteins by culturing said recombinant yeast cells.
Claims8
A recombinant expression and secretion vector for Saccharomyces cerevisiae comprising:
- a polynucleotide encoding a secretion signal peptide which comprises the amino acid sequence of SEQ ID NO:1 or 2;
- a DNA sequence which promotes transcription in a Saccharomyces cerevisiae cell, located upstream from the polynucleotide encoding the secretion signal peptide;
- a DNA sequence encoding lipocortin-I or interleukin-2 operably linked to the polynucleotide encoding the secretion signal peptide;
- and a transcription terminator sequence located downstream from the DNA sequence encoding lipocortin-I or interleukin-2.
Dependencies are inferred from the claim text.