Method of screening compounds which inhibit P. gingivalis lipopolysaccharide from inhibiting the extravasation of leukocytes
AssigneeBristol-Myers Squibb Company
InventorRichard P. Darveau
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Abstract
The lipopolysaccharide of bacteria associated with chronic inflammatory diseases is unable to induce expression of leukocyte adhesion molecules, or selectins, on endothelial cells, and is also capable of inhibiting the induction of selectin expression by bacteria normally associated with acute endotoxin disease. New approaches to treatment of these diseases, and the diagnosis of susceptibility to chronic bacterial-associated inflammatory diseases, are provided.
Claims6
A method for screening compounds which inhibit the ability of P. gingivalis lipopolysaccharide to inhibit the extravasation of leukocytes from the vascular endothelium to gingival tissues, comprising
- contacting cells which are capable of expressing a selectin molecule with P. gingivalis lipopolysaccharide in the presence and absence of the compound being screened for the ability to inhibit P. gingivalis lipopolysaccharide-induced inhibition of selectin expression;
- stimulating expression of the selectin molecule by the cells;
- and
- measuring the expression of the selectin molecule by the cells in the presence or absence of said compound and therefrom determining the ability of said compound to inhibit P. gingivalis lipopolysaccharide-induced inhibition of selectin expression.
Dependencies are inferred from the claim text.