# US 5,710,367 A · Apomictic maize

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- Number: US 5,710,367 A
- Document ID: US5710367A
- Status: Granted
- Kind: A
- Application number: 08/532,904
- Filed: 1995-09-22
- Granted: 1998-01-20
- Earliest claimed: 1995-09-22
- Assignees: The United States of America as represented by the Secretary of Agriculture
- Inventors: Bryan K. Kindiger; Victor Sokolov
- Web page: https://patentgrep.com/patent/US5710367A

## CPC

- A01H 6/4684: {Zea mays [maize]}
- A01H 5/10: Seeds
- C12N 15/8287: {for fertility modification, e.g. apomixis}
- Y10S 47/01: Methods of plant-breeding and including chromosome multiplication
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Apomictic maize/Tripsacum hybrids having a ratio of maize chromosomes: Tripsacum chromosomes of at least 30:9 have been developed. These hybrids are useful for introgressing diplosporous apomictic reproduction into a maize background toward the ultimate goal of establishing immortalized commercial lines of apomictic maize having stably inherited characteristics without the need for continuously producing hybrid seed by repeated crossings of selected parental lines. DNA primers for use in assaying maize/Tripsacum hybrids for apomictic reproduction behavior are provided.

## Claims

### Claim 1

Apomictic maize having at least one dominant gene N for controlling nonreduction of the egg and at least one dominant gene A for controlling apomictic development of the egg.

### Claim 2 (depends on 1)

The apomictic maize of claim 1, wherein the genome has exactly one dominant gene A and at exactly one dominant gene N.

### Claim 3 (depends on 1)

The apomictic maize of claim 1 having a karyotype comprising the long arm of Tripsacum dactyloides chromosome number 16 carrying the alleles for apomixis.

### Claim 4 (depends on 1)

The apomictic maize of claim 1 characterized by a Mz6-Tr16 translocation.

### Claim 5

An apomictic maize/Tripsacum hybrid having a karyotype wherein the ratio of maize chromosomes: Tripsacum chromosomes is at least 30:9, and wherein said hybrid has at least one dominant gene N for controlling nonreduction of the egg and at least one dominant gene A for controlling apomictic development of the egg.

### Claim 6 (depends on 5)

The hybrid of claim 5, wherein the karyotype comprises from 30-70 chromosomes of maize and 9 chromosomes of Tripsacum.

### Claim 7 (depends on 1)

The maize of claim 1 having at least one marker on a chromosome carrying a gene for apomixis, which marker is amplified in a polymerase chain reaction by a primer, wherein said primer is selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, and SEQ ID NO 7.

### Claim 8 (depends on 1)

Seed of the maize of claim 1.

### Claim 9 (depends on 2)

Seed of the maize of claim 2.

### Claim 10 (depends on 3)

Seed of the maize of claim 3.

### Claim 11 (depends on 4)

Seed of the maize of claim 4.

### Claim 12 (depends on 5)

Seed of the hybrid of claim 5.

### Claim 13 (depends on 6)

Seed of the hybrid of claim 6.

### Claim 14

A method of increasing the complement of maize chromosomes in a maize/Tripsacum hybrid comprising backcrossing the hybrid with a sexually reproducing maize line and selecting for a B.sub.III -derived hybrid, wherein said maize/Tripsacum hybrid has a ratio of maize chromosomes: Tripsacum chromosomes of at least 30:9.

### Claim 15 (depends on 14)

The method of claim 14 wherein said maize/Tripsacum hybrid comprises 30 chromosomes of Zea mays and 9 chromosomes of Tripsacum dactyloides.

### Claim 16 (depends on 15)

The method of claim 15 wherein said maize/Tripsacum hybrid is ATCC Accession No, 97233.

### Claim 17 (depends on 15)

The method of claim 15 wherein said maize/Tripsacum hybrid comprises a Mz6-Tr16 translocation.

### Claim 18

A method of increasing the complement of maize chromosomes in a maize/Tripsacum hybrid comprising backcrossing the hybrid with a sexually reproducing maize line, selecting for an individual having a Mz6-Tr16 translocation, backcrossing that individual with a sexually reproducing maize line and selecting for a B.sub.III -derived hybrid.

### Claim 19 (depends on 18)

The method of claim 18 wherein said maize/Tripsacum hybrid comprises 30 chromosomes of Zea mays and 9 chromosomes of Tripsacum dactyloides.

### Claim 20 (depends on 19)

The method of claim 19 wherein said maize/Tripsacum hybrid is ATCC Accession No, 97233.

### Claim 21

A method of introgressing apomixis into a maize that reproduces sexually comprising transferring into said maize both the dominant allele N responsible for non-reduction and the dominant allele A responsible for apomictic development of an unreduced egg.

### Claim 22

An isolated region of the long arm of Tripsacum dactyloides chromosome 14 comprising the dominant allele N responsible for non-reduction and the dominant allele A responsible for apomictic development of an unreduced egg.

### Claim 23 (depends on 1)

The apomictic maize of claim 1, wherein said N and A genes are associated with the Mz6-chromosome.

### Claim 24 (depends on 23)

Seed of the maize of claim 23.

### Claim 25 (depends on 5)

The apomictic maize/Tripsacum hybrid of claim 5 characterized by a Mz6-Tr16 translocation.

### Claim 26 (depends on 5)

The apomictic maize/Tripsacum hybrid of claim 5, wherein said N and A genes are on a maize chromosome.

### Claim 27 (depends on 25)

Seed of the hybrid of claim 25.

### Claim 28 (depends on 26)

Seed of the hybrid of claim 26.