# US 5,710,266 A · Nucleic acid encoding an interleukin 4 signal transducer

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- Number: US 5,710,266 A
- Document ID: US5710266A
- Status: Granted
- Kind: A
- Application number: 08/781,890
- Filed: 1997-01-05
- Granted: 1998-01-20
- Earliest claimed: 1994-07-05
- Assignees: Tularik Inc.
- Inventors: Steven L. McKnight; Jinzhao Hou
- Web page: https://patentgrep.com/patent/US5710266A

## CPC

- C07K 14/4705: {stimulating, promoting or activating activity}
- C07K 14/705: Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)}
- C12Q 1/6897: involving reporter genes operably linked to promoters
- G01N 33/6869: {Interleukin}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The invention provides methods and compositions for identifying pharmacological agents useful in the diagnosis or treatment of disease associated with the expression of a gene modulated by an interleukin 4 signal transducer and activator of transcription, IL-4 Stat. IL-4 Stat peptides and IL-4 receptor peptides and nucleic acids encoding such peptides find therapeutic uses. The subject compositions include IL-4 Stat and IL-4 receptor proteins, portions thereof, nucleic acids encoding them, and specific antibodies. The disclosed pharmaceutical screening methods are particularly suited to high-throughput screening where one or more steps are performed by a computer controlled electromechanical robot comprising an axial rotatable arm.

## Claims

### Claim 1

An isolated nucleic acid encoding an interleukin-4 signal transducer and activator of transcription (IL-4 STAT) protein wherein said protein:
(a) selectively binds a transcription factor binding site having a sequence selected from the group consisting of SEQ ID NO: 03, SEQ ID NO: 04, SEQ ID NO: 05, SEQ ID NO: 06, SEQ ID NO: 07, SEQ ID NO: 08 or SEQ ID NO: 09; and
(b) selectively binds an IL-4 receptor peptide having a sequence defined by SEQ ID NO: 10 or SEQ ID NO: 11; and
(c) is encoded by a DNA which hybridizes with SEQ ID NO:01 under high stringency conditions.

### Claim 2

An isolated nucleic acid encoding an interleukin-4 signal transducer and activator of transcription (IL-4 STAT) protein, wherein said protein comprises at least one of the amino acid sequences selected from the group consisting of SEQ ID NO: 02, residues 1 to 40 and SEQ ID NO:02, residues 401 to 650.

### Claim 3

An isolated nucleic acid encoding an interleukin-4 signal transducer and activator of transcription (IL-4 STAT) protein, wherein said protein comprises the amino acid sequence of SEQ ID NO:2.