# US 5,710,265 A · Gene encoding melanocortin-5 receptor and methods of use > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,710,265 A - Document ID: US5710265A - Status: Granted - Kind: A - Application number: 08/672,109 - Filed: 1996-06-27 - Granted: 1998-01-20 - Earliest claimed: 1994-02-17 - Assignees: The Regents of the University of Michigan - Inventors: Tadataka Yamada; Ira Gantz - Web page: https://patentgrep.com/patent/US5710265A ## CPC - C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes - C07K 14/723: {G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor} - A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) - C12Q 2600/158: Expression markers - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract Genes encoding melanocortin receptors have been identified, isolated, cloned and localized to their chromosomal positions. These genes have been used to transfect mammalian cells lacking endogenous melanocortin receptors to induce expression. Additionally, melanocortin receptor binding, secondary signalling, and tissue distribution has been characterized. The genes and their gene products may therefore be used to to provide therapeutic vehicles for the treatment of processes involving the function of melanocortin receptors. ## Claims ### Claim 1 An isolated nucleic acid molecule encoding the amino acid sequence of Sequence Listing ID No. 10. ### Claim 2 (depends on 1) A vector comprising the nucleic acid molecule of claim 1. ### Claim 3 (depends on 2) A mammalian cell comprising the vector of claim 2. ### Claim 4 (depends on 2) A bacterial cell comprising the vector of claim 2. ### Claim 5 (depends on 2) A yeast cell comprising the vector of claim 2. ### Claim 6 An isolated nucleic acid molecule comprising a nucleotide sequence which hybridizes to the nucleic acid comprising the nucleotide sequence of Sequence Listing ID No. 9 in 6.times.SSC at 42.degree. C. followed by washing with 1.times.SSC at 55.degree. C. wherein said isolated nucleic acid molecule encodes a melanocortin-5 receptor. ### Claim 7 (depends on 6) A vector comprising the nucleic acid molecule of claim 6. ### Claim 8 (depends on 7) A mammalian cell comprising the vector of claim 7. ### Claim 9 (depends on 7) A bacterial cell comprising the vector of claim 7. ### Claim 10 (depends on 7) A yeast cell comprising the vector of claim 7. ### Claim 11 An isolated nucleic acid molecule comprising a nucleotide sequence which hybrizes to the nucleic acid comprising the complementary nucleotide sequence of Sequence Listing ID No. 9 in 6.times.SSC at 42.degree. C. followed by washing with 1.times.SSC at 55.degree. C., wherein said isolated nucleic acid molecule encodes a melanocortin-5 receptor. ### Claim 12 (depends on 11) A vector comprising the nucleic acid molecule of claim 11. ### Claim 13 (depends on 12) A mammalian cell comprising the vector of claim 12. ### Claim 14 (depends on 12) A bacterial cell comprising the vector of claim 12. ### Claim 15 (depends on 12) A yeast cell comprising the vector of claim 12. ### Claim 16 A method for assaying the binding of a ligand to a melanocortin-5 receptor comprising the steps of: a) transfecting a cell which does not endogenously express melanocortin-5 receptor with a vector comprising an isolated nucleic acid molecule comprising a nucleotide sequence which hybridizes to the nucleic acid comprising the nucleotide sequence of Sequence Listing ID No. 9 in 6.times.SSC at 42.degree. C. followed by washing with 1.times.SSC at 55.degree. C., wherein said isolated nucleic acid molecule encodes a melanocortin-5 receptor, under conditions favorable for expression of melanocortin-5 receptor; b) contacting the cell with a ligand; and c) detecting ligand binding to the expressed melanocortin-5 receptor.