Mediators to oxidoreductase enzymes
AssigneeMedisense, Inc.
InventorsNigel Forrow, Stephen Walters
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Abstract
Half-sandwich complexes of transition metals having a single .pi.-ligand with delocalised .pi. bonding are of use in mediating electron transfer between an electrode and a redox protein, for example an oxidoreductase enzyme such as in an amperometric enzyme sensor. Suitable compounds include complexes of the general formula: (.pi.-ligand)M(not-.pi.-ligand).sub.n wherein the complex is charged or neutral, .pi.-ligand represents the single .pi.-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not .pi.-ligands, and the n not-.pi.-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transitional metal M.
Claims36
A process of mediating electron transfer between an electrode and an oxidoreductase enzyme or other electron transfer redox protein using a mediator compound, wherein said mediator compound is a half-sandwich complex of a transition metal, said complex having only a single .pi.-ligand with delocalised .pi. bonding and having the general formula:
- (.pi.-ligand)M(not-.pi.-ligand).sub.n
- wherein the complex may be charged or neutral, .pi.-ligand represents the single .pi.-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not .pi.-ligands, and the n not-.pi.-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transition metal M.
An amperometric enzyme sensor for an analyte, which sensor employs a transition metal half-sandwich compound as the mediator, said half-sandwich transition metal half-sandwich compound including a transition metal complexed to only a single .pi. ligand with delocalized .pi. bonding and having the general formula:
- (.pi.-ligand)M(not-.pi.-ligand).sub.n
- wherein the complex may be charged or neutral, .pi.-ligand represents the single-.pi.-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not .pi. ligands, and the n not-.pi.-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transition metal M.
A disposable dry strip test element for an amperometric enzyme sensor, said disposable test element carrying a working electrode, test reagents comprising at least an oxidoreductase enzyme and a half-sandwich complex of a transition metal as the mediator for generation of a current indicative of the level of analyte, and a reference electrode, said complex having a single .pi. ligand with delocalized .pi. bonding and having the general formula:
- (.pi.-ligand)M(not-.pi.-ligand).sub.n
- wherein the complex may be charged or neutral, .pi.-ligand represents the single-.pi.-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not .pi. ligands, and the n not-.pi.-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transition metal M.
An electrically conductive printing ink which includes a half-sandwich complex of a transition metal as the mediator, said half-sandwich complex having a single .pi. ligand with delocalized .pi. bonding and having the general formula:
- (.pi.-ligand)M(not-.pi.-ligand).sub.n
- wherein the complex may be charged or neutral, .pi.-ligand represents the single-.pi.-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not .pi. ligands, and the n not-.pi.-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transitional metal M.
Dependencies are inferred from the claim text.