# US 5,708,158 A · Nuclear factors and binding assays

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- Number: US 5,708,158 A
- Document ID: US5708158A
- Status: Granted
- Kind: A
- Application number: 08/818,823
- Filed: 1997-03-14
- Granted: 1998-01-13
- Earliest claimed: 1994-07-05
- Assignees: Tularik Inc.
- Inventors: Timothy Hoey
- Web page: https://patentgrep.com/patent/US5708158A

## CPC

- G01N 33/5091: {for testing the pathological state of an organism}
- C07K 14/4702: {Regulators; Modulating activity}
- G01N 33/5008: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics}
- G01N 33/5011: {for testing antineoplastic activity}
- G01N 33/505: {involving T-cells}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The invention provides methods and compositions for identifying pharmacological agents useful in the diagnosis or treatment of disease associated with the expression of a gene modulated by a transcription complex containing at least a human nuclear factor of activated T-cells (hNFAT). The materials include a family of hNFAT proteins, active fragments thereof, and nucleic acids encoding them. The methods are particularly suited to high-throughput screening where one or more steps are performed by a computer controlled electromechanical robot comprising an axial rotatable arm.

## Claims

### Claim 1

An isolated nucleic acid encoding a human nuclear factor of activated T-cells, hNFAT, protein comprising hNFATp.sub.1 (SEQ ID NO:2), hNFATp.sub.2 (SEQ ID NO:2, residues 220-921), hNFAT3 (SEQ ID NO:6), hNFAT4b (SEQ ID NO:10) or hNFAT4c (SEQ ID NO:12).

### Claim 2 (depends on 1)

The isolated nucleic acid of claim 1, wherein said protein comprises hNFATp.sub.1 (SEQ ID NO:2).

### Claim 3 (depends on 1)

The isolated nucleic acid of claim 1, wherein said protein comprises hNFATp.sub.2 (SEQ ID NO:2, residues 220-921).

### Claim 4 (depends on 1)

The isolated nucleic acid of claim 1, wherein said protein comprises hNFAT3 (SEQ ID NO:6).

### Claim 5 (depends on 1)

The isolated nucleic acid of claim 1, wherein said protein comprises hNFAT4b (SEQ ID NO:10).

### Claim 6 (depends on 1)

The isolated nucleic acid of claim 1, wherein said protein comprises hNFAT4c (SEQ ID NO:12).

### Claim 7

An isolated nucleic acid encoding an hNFAT protein, said nucleic acid comprising SEQ ID NO:1, SEQ ID NO:5, SEQ ID NQ:9, SEQ ID NO:11 or nucleotides 1-356 and 868-3478 of SEQ ID NO:1.

### Claim 8 (depends on 7)

The isolated nucleic acid of claim 7, wherein said nucleic acid comprises SEQ ID NO:1.

### Claim 9 (depends on 7)

The isolated nucleic acid of claim 7, wherein said nucleic acid comprises nucleotides 1-356 and 868-3478 of SEQ ID NO:1.

### Claim 10 (depends on 7)

The isolated nucleic acid of claim 7, wherein said nucleic acid comprises SEQ ID NO:5.

### Claim 11 (depends on 7)

The isolated nucleic acid of claim 7, wherein said nucleic acid comprises SEQ ID NO:9.

### Claim 12 (depends on 7)

The isolated nucleic acid of claim 7, wherein said nucleic acid comprises SEQ ID NO:1.