# US 5,708,157 A · Secreted proteins and polynucleotides encoding them

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- Number: US 5,708,157 A
- Document ID: US5708157A
- Status: Granted
- Kind: A
- Application number: 08/686,878
- Filed: 1996-07-26
- Granted: 1998-01-13
- Earliest claimed: 1996-07-26
- Assignees: Genetics Institute, Inc.
- Inventors: Kenneth Jacobs; John M. McCoy; Edward R. LaVallie; Lisa A. Racie; David Merberg; Maurice Treacy; Cheryl Evans; Vikki Spaulding
- Web page: https://patentgrep.com/patent/US5708157A

## CPC

- C07K 14/47: from mammals
- A61P 3/02: Nutrients, e.g. vitamins, minerals
- A61P 7/02: Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- A61P 7/08: Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock (artificial tears A61P27/04)
- A61P 35/00: Antineoplastic agents
- A61P 37/00: Drugs for immunological or allergic disorders
- Y10S 530/85: Reproductive organs or embryos
- Y10S 530/851: Placenta; amniotic fluid
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Novel polynucleotides and the proteins encoded thereby are disclosed.

## Claims

### Claim 1

An isolated polynucleotide selected from the group consisting of:
(a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:1;
(b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:1 from nucleotide 218 to nucleotide 628;
(c) a polynucleotide comprising the nucleotide sequence of the full length protein coding sequence of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115;
(d) a polynucleolide encoding the full length protein encoded by the cDNA insert of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115;
(e) a polynucleotide comprising the nucleotide sequence of the mature protein coding sequence of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115;
(f) a polynucleotide encoding the mature protein encoded by the cDNA insert of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115;
(g) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:2, said sequence of SEQ ID NO:2 being the sequence encoded by the cDNA insert of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115;
(h) a polynucleotide which is an allelic variant of a polynucleotide of (a)-(f) above; and
(i) a polynucleotide capable of hybridizing under conditions of incubation at 65.degree. C. in 6.times.SSC/0.5% SDS, followed by washing with 2.times.SSC/0.5% SDS at room temperature, with a polynucleotide consisting of the nucleotide sequence of SEQ ID NO:1 from nucleotide 218 to nucleotide 628.

### Claim 2 (depends on 1)

An isolated polynucleotide of claim 1 wherein said polynucleotide is operably linked to an expression control sequence.

### Claim 3 (depends on 2)

A host cell transformed with a polynucleotide of claim 2.

### Claim 4 (depends on 3)

The host cell of claim 3, wherein said cell is a mammalian cell.

### Claim 5 (depends on 2, 3)

A process for producing a protein encoded by a polynucleotide of claim 2, which process comprises:
(a) growing a culture of the host cell of claim 3 in a suitable culture medium; and
(b) purifying said protein from the culture.

### Claim 6 (depends on 5)

A protein produced according to the process of claim 5.

### Claim 7 (depends on 6)

The protein of claim 6 comprising a mature protein.

### Claim 8

An isolated protein, wherein said protein comprises an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence of SEQ ID NO:2, said sequence of SEQ ID NO:2 being the sequence encoded by the cDNA insert of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115;
(b) the amino acid sequence of SEQ ID NO:2 from amino acid 27 to amino acid 82; and
(c) the amino acid sequence encoded by the cDNA insert of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115;
the protein being substantially free from other mammalian proteins.

### Claim 9 (depends on 8)

The protein of claim 8, wherein said protein comprises the amino acid sequence of SEQ ID NO:2, said sequence of SEQ ID NO:2 being the sequence encoded by the cDNA insert of clone AJ26.sub.-- 3 deposited under accession number ATCC. 98115.

### Claim 10 (depends on 8)

The protein of claim 8, wherein said protein comprises the amino acid sequence of SEQ ID NO:2 from amino acid 27 to amino acid 82.

### Claim 11 (depends on 8)

A composition comprising protein of claim 8, and a pharmaceutically acceptable carrier.

### Claim 12

An isolated gene corresponding to the cDNA sequence of SEQ ID NO:1 and SEQ ID NO:3.

### Claim 13 (depends on 1)

The polynucleotide of claim 1 wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO:1.

### Claim 14 (depends on 1)

The polynucleotide of claim 1 wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO:1 from nucleotide 218 to nucleotide 628.

### Claim 15 (depends on 1)

The polynucleotide of claim 1 wherein said polynucleotide comprises the nucleotide sequence of the full length protein coding sequence of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115.

### Claim 16 (depends on 1)

The polynucleotide of claim 1 wherein said polynucleotide comprises a polynucleotide encoding the full length protein encoded by the cDNA insert of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115.

### Claim 17 (depends on 1)

The polynucleotide of claim 1 wherein said polynucleotide comprises a polynucleotide comprising the nucleotide sequence of the mature protein coding sequence of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115.

### Claim 18 (depends on 1)

The polynucleotide of claim 1 wherein said polynucleotide comprises a polynucleotide encoding the mature protein encoded by the cDNA insert of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115.

### Claim 19 (depends on 1)

The polynucleotide of claim 1 wherein said polynucleotide comprises a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:2, said sequence of SEQ ID NO:2 being the sequence encoded by the cDNA insert of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115.

### Claim 20 (depends on 1)

The polynucleotide of claim 1 wherein said polynucleotide comprises a polynucleotide capable of hybridizing under conditions of incubation at 65.degree. C. in 6.times.SSC/0.5% SDS, followed by washing with 2.times.SSC/0.5% SDS at room temperature, with a polynucleotide consisting of the nucleotide sequence of SEQ ID NO:1 from nucleotide 218 to nucleotide 628.

### Claim 21 (depends on 8)

The protein of claim 8 wherein said protein comprises the amino acid sequence encoded by the cDNA insert of clone AJ26.sub.-- 3 deposited under accession number ATCC 98115.