# US 5,708,144 A · Process for the purification of natural pulmonary surfactant material using supercritical fluids

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- Number: US 5,708,144 A
- Document ID: US5708144A
- Status: Granted
- Kind: A
- Application number: 08/424,503
- Filed: 1995-05-22
- Granted: 1998-01-13
- Earliest claimed: 1992-11-27
- Assignees: Chiesi Farmaceutici S.p.A.
- Inventors: Paolo Chiesi; Paolo Ventura; Roberto Pighi; Vittorino Servadio; Walter Recchia
- Web page: https://patentgrep.com/patent/US5708144A

## CPC

- C07K 14/785: Alveolar surfactant peptides; Pulmonary surfactant peptides
- B01D 11/0203: {with a supercritical fluid}
- Y02P 20/54: using solvents, e.g. supercritical solvents or ionic liquids
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

A process for purifying natural pulmonary surfactant by dispersing animal lung tissue on an inert carrier and extracting with a supercritical fluid.

## Claims

### Claim 1

A process for extracting natural pulmonary surfactant from an animal lung tissue, comprising the steps of:
dispersing said animal lung tissue on an inert carrier material, and
extracting said animal lung tissue with a supercritical fluid.

### Claim 2 (depends on 1)

The process of claim 1, wherein said animal lung tissue material is in the form of a paste.

### Claim 3 (depends on 1)

The process of claim 1, wherein said supercritical fluid is carbon dioxide.

### Claim 4 (depends on 1)

The process of claim 1, where said inert carrier material is glass beads or diatomaceous earth.

### Claim 5 (depends on 1)

The process of claim 1, comprising a plurality of said extracting steps.

### Claim 6 (depends on 1)

A natural pulmonary surfactant obtained by the process of claim 1.

### Claim 7 (depends on 1)

The process of claim 1, wherein the weight ratio of inert carrier material to animal lung tissue material ranges from 0.5:1 to 3:1.

### Claim 8 (depends on 7)

The process of claim 7, wherein said ratio is 1:1.

### Claim 9 (depends on 1)

The process of claim 1, wherein said extracting step is carried out at a temperature of 35.degree. to 55.degree. C. under a pressure from 180 to 300 atmospheres and for a time from 3 to 9 hours.

### Claim 10 (depends on 9)

The process of claim 9, wherein said extracting step is carried out at a temperature of 40.degree. C. under a pressure of 200 atmospheres, and for a time of 7 hours.

### Claim 11 (depends on 1)

The process of claim 1, wherein said extracting step comprises extracting with a mixture of said supercritical fluid and a cosolvent.

### Claim 12 (depends on 11)

The process of claim 11, wherein said cosolvent is chloroform.