# US 5,708,139 A · Pseudomonas syringae pv syringae hrpZ gene

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- Number: US 5,708,139 A
- Document ID: US5708139A
- Status: Granted
- Kind: A
- Application number: 08/062,024
- Filed: 1993-05-17
- Granted: 1998-01-13
- Earliest claimed: 1993-05-17
- Assignees: Cornell Research Foundation, Inc.
- Inventors: Alan Collmer; Sheng-Yang He
- Web page: https://patentgrep.com/patent/US5708139A

## CPC

- C07K 14/21: from Pseudomonadaceae (F)
- C12N 15/8281: {for bacterial resistance}
- Y10S 435/874: Pseudomonas
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

The nucleic acid and amino acid sequences for proteinaceous elicitors of the plant defense reaction known as the hypersensitive response against Pseudomonas syringae are described along with method for preparation.

## Claims

### Claim 1

An isolated Pseudomonas syringae protein capable of eliciting a hypersensitive response when said protein is introduced into tissue of a plant with which a Pseudomonas syringae pathogen is incompatible, under normal plant growth conditions, wherein the hypersensitive response is characterized by localized cell death in the plant tissue where the protein is introduced, the protein is glycine rich, lacks cysteine, is heat stable, lacks amino-terminal signal peptides, and is hydrophilic.

### Claim 2 (depends on 1)

An isolated Pseudomonas syringae protein according to claim 1, wherein said protein comprises the amino acid sequence Gly Gly Gly Leu Gly Thr Pro.

### Claim 3 (depends on 1)

An isolated Pseudomonas syringae protein according to claim 1, wherein said protein comprises the amino acid sequence Gln Thr Gly Thr.

### Claim 4 (depends on 1)

An isolated protein according to claim 1, wherein the protein has a molecular weight of 34.7 kDa.

### Claim 5 (depends on 4)

An isolated protein according to claim 4, wherein the protein has an amino acid sequence of SEQ. ID. No. 5.

### Claim 6 (depends on 1)

An isolated protein according to claim 1, wherein the protein lacks tyrosine.

### Claim 7 (depends on 1)

An isolated protein according to claim 1, wherein the protein has repeat amino acid sequences of SEQ. ID. Nos. 1 and 2.

### Claim 8 (depends on 1)

An isolated protein according to claim 1, wherein the protein is purified.

### Claim 9 (depends on 1)

An isolated protein according to claim 1, wherein the protein is recombinant.

### Claim 10 (depends on 1)

An isolated protein fragment comprising a 25.1 carboxyl terminal fragment of the protein of claim 1.

### Claim 11 (depends on 1)

An isolated protein fragment of the protein of claim 1 comprising amino acids 194 to 341 of SEQ ID NO:5.