# US 5,707,963 A · Methods of using growth inhibitory peptides > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,707,963 A - Document ID: US5707963A - Status: Granted - Kind: A - Application number: 08/636,386 - Filed: 1996-04-23 - Granted: 1998-01-13 - Earliest claimed: 1994-10-26 - Assignees: Health Research, Inc. - Inventors: Gerald J. Mizejewski - Web page: https://patentgrep.com/patent/US5707963A ## CPC - C07K 14/475: Growth factors; Growth regulators - A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract The subject invention provides non-naturally occurring peptides capable of inhibiting growth factor-stimulated growth of cells. The peptide can be utilized to inhibit growth factor-stimulated growth, such growth factors including, for example, gonadotropins, peptide hormones, synthetic growth factors, and ligands, the ligand having a receptor that is a member of the steroid/thyroid hormone/vitamin receptor superfamily. Also provided are DNA sequences encoding the peptides and methods of producing and using the peptides. ## Claims ### Claim 1 A method of inhibiting growth factor-stimulated growth of cells, said growth factor-stimulated growth being stimulated by a growth factor selected from the group consisting of estrogen, diethylstilbestrol, hydrocortisone, and human chorionic gonadotropin, said method comprising selecting a sample having cells therein capable of growth factor-stimulated growth, and contacting said sample with a peptide so as to inhibit growth factor-stimulated growth of said cells, wherein said peptide is a non-naturally occurring peptide having an amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:8. ### Claim 2 A method of inhibiting growth factor-stimulated growth of cells, said growth factor-stimulated growth being stimulated by a growth factor selected from the group consisting of estrogen, diethylstilbestrol, hydrocortisone, and human chorionic gonadotropin, said method comprising selecting a sample having cells therein capable of growth factor-stimulated growth, and contacting said sample with a peptide so as to inhibit growth factor-stimulated growth of said cells, wherein said peptide is a non-naturally occurring peptide having an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, and SEQ ID NO:11. ### Claim 3 (depends on 1) The method of claim 1 wherein said growth factor is estrogen and said cells are uterus cells. ### Claim 4 (depends on 1) The method of claim 1 wherein said growth factor is diethylstilbestrol and said cells are uterus cells. ### Claim 5 (depends on 1) The method of claim 1 wherein said growth factor is hydrocortisone and said cells are spleen cells. ### Claim 6 (depends on 1) The method of claim 1 wherein said growth factor is human chorionic gonadotropin and said cells are ovary cells. ### Claim 7 (depends on 1) The method of claim 1 wherein said peptide is methylated, amidated, or biotinylated. ### Claim 8 (depends on 2) The method of claim 2 wherein said growth factor is estrogen and said cells are uterus cells. ### Claim 9 (depends on 2) The method of claim 2 wherein said growth factor is diethylstilbestrol and said cells are uterus cells. ### Claim 10 (depends on 2) The method of claim 2 wherein said growth factor is hydrocortisone and said cells are spleen cells. ### Claim 11 (depends on 2) The method of claim 2 wherein said growth factor is human chorionic gonadotropin and said cells are ovary cells. ### Claim 12 (depends on 2) The method of claim 2 wherein said peptide is methylated, amidated, or biotinylated.