# US 5,707,861 A · Disintegrator of living cells

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- Number: US 5,707,861 A
- Document ID: US5707861A
- Status: Granted
- Kind: A
- Application number: 08/528,115
- Filed: 1995-09-14
- Granted: 1998-01-13
- Earliest claimed: 1995-09-14
- Assignees: Scientific Industries, Inc.
- Inventors: Michael Sherman; Yury Sherman; Katerina Sherman
- Web page: https://patentgrep.com/patent/US5707861A

## CPC

- C12M 23/48: {Holding appliances; Racks; Supports (holding devices for laboratory apparatus B01L9/00)}
- C12M 47/06: {Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material (lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003)}
- Y10S 435/809: Incubators or racks or holders for culture plates or containers
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

An auxiliary holder of tubes intended for disintegration of living cells, such as yeast cells, in the presence of glass beads, as well as for mixing substances and pellet resuspension. The holder is installed on a vibrator. Due to specially-made gaps between the tubes and the main parts of the holder the processed cells and substances are subjected to a complex impact of vibration created by the vibrator and shocks created by the holder. The holder strongly enhances the effectiveness of the vibrator. During the disintegrating process the presence of a researcher is not required.

## Claims

### Claim 1

A tube holder for holding at least one tube, the holder comprising:
a shaft having means for coupling the shaft to a vibrator;
a driving member coupled to the shaft and including at least one first hole through which a first portion of the tube can pass, the hole being shaped such that the tube is engaged by the driving member at least while the driving member is vibrated by the shaft; and
a shocking-member spaced from the driving member along the length of the tube shaped to be moveable with respect to the shaft, the shocking member including at least one second hole through which a second portion of the tube can pass, the second hole being larger in cross-section than the tube and sized so that as the tube is vibrated by the driving member, the tube may impact on the shocking member.

### Claim 2

The tube holder of aim 1, wherein the first hole is sized larger in cross section than the first portion of the tube to produce a gap between the driving member and the first portion of the tube and the first hole is small enough in cross section that the driving member may vibrate the tube.

### Claim 3 (depends on 2)

The tube holder of claim 2, wherein the first hole is smaller than a third support portion of the tube such that the support portion of the tube is supported by the driving member.

### Claim 4 (depends on 2)

The tube holder of claim 2, wherein the shaft is shaped for supporting the shocking member spaced from the driving member.

### Claim 5 (depends on 2)

The tube holder of claim 2, wherein the shocking member includes a mounting hole through which the shaft passes, and the mounting hole is larger in cross-section than the portion of the shaft which passes through the mounting hole producing a gap between the shaft and the shocking member such that the shocking member is movingly coupled with the shaft.

### Claim 6 (depends on 5)

The tube holder of claim 5, wherein the shaft includes a shoulder thereon for supporting the shocking member spaced from the driving member.

### Claim 7 (depends on 6)

The tube holder of claim 6, wherein the mounting hole is smaller in cross-section than the shoulder.

### Claim 8 (depends on 7)

The tube holder of claim 7, wherein the center of the shaft is not coincident with the center of the mounting hole.

### Claim 9 (depends on 5)

The tube holder of claim 5, wherein each of the driving member and the shocking member is in the shape of a respective disk, and each has a respective plurality of the first and second holes arranged in respective sets of a first and second hole for receiving a tube.

### Claim 10 (depends on 9)

The tube holder of claim 9, wherein the pluralities of holes are disposed about the peripheries of the respective members.

### Claim 11 (depends on 5)

The tube holder of claim 5, wherein the shocking member is spaced from the driving member approximately 0.85 of the length of the tube.

### Claim 12 (depends on 1)

The tube holder of claim 1, further comprising a cap near the driving member for preventing the tube from disengaging from the driving member during vibration.

### Claim 13 (depends on 12)

The tube holder of claim 12, wherein the cap is coupled to the shaft.

### Claim 14 (depends on 13)

The tube holder of claim 13, wherein the cap is removable.

### Claim 15 (depends on 12)

The tube holder of claim 12, further comprising a fixed column and a movable lever coupled to the column, the cap being coupled to the lever and biased against the shaft.

### Claim 16 (depends on 15)

The tube holder of claim 15, further comprising a spring disposed in the column for biasing the lever and the cap toward the shaft.

### Claim 17

A tube holder for a plurality of flanged tubes for disintegrating living cells, mixing substances, and pellet resuspension by vibration, comprising: a shaft, a driving wheel, and a shocking wheel;
the shaft having a shoulder; means for affixing the shaft to a vibrator;
the driving wheel being coupled to the shaft, the driving wheel having a plurality of peripheral holes the tubes can pass through, wherein the diameter of the peripheral holes is larger than the diameter of the tubes at the point the tubes pass through the holes, producing a gap between each tube and the driving wheel, the diameter of the peripheral holes of the driving wheel is less than the diameter of the flanges of the tubes, so that the tubes rest upon the driving wheel;
the shocking wheel resting upon the shoulder of the shaft a distance from the driving wheel which is less than the length of the tubes, the shocking wheel having a central hole the diameter of which is larger than the diameter of the shaft above the shoulder and less than the diameter of the shoulder, producing a gap between the shaft and the shocking wheel and so that the shocking wheel is free to move relative to the shaft, the shocking wheel having a plurality of peripheral holes which correspond to the holes of the driving wheel, wherein the diameter of the peripheral holes of the shocking wheel is larger than the diameter of the tubes at the point the tubes pass through the holes, producing a gap between each tube and the shocking wheel.

### Claim 18 (depends on 17)

The tube holder of claim 17, including a removable cap positioned above the wheels for preventing the tubes from being displaced from the holder when subjected to vibrations and shocks.

### Claim 19 (depends on 17)

The tube holder of claim 17, wherein the central point of the shaft does not coincide with the central point of the central hole of the shocking wheel.

### Claim 20 (depends on 17)

The tube holder of claim 17, further including cantilever means for selectively providing vertical axial pressure on the shaft.