# US 5,707,843 A · Lactose enzyme compositions and method > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,707,843 A - Document ID: US5707843A - Status: Granted - Kind: A - Application number: 08/707,732 - Filed: 1996-09-03 - Granted: 1998-01-13 - Earliest claimed: 1996-09-03 - Inventors: Woodrow C. Monte - Web page: https://patentgrep.com/patent/US5707843A ## CPC - A23G 9/40: characterised by the dairy products used - A23C 9/1206: {Lactose hydrolysing enzymes, e.g. lactase, beta-galactosidase} - A23G 9/34: characterised by carbohydrates used, e.g. polysaccharides (characterised by the dairy products used A23G9/40) - A23G 9/363: {containing microorganisms, enzymes} - A23G 9/52: Liquid products; Solid products in the form of powders, flakes or granules for making liquid products {; Finished or semi-finished solid products, frozen granules} - C12N 9/2471: {Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase} - C12N 9/98: Preparation of granular or free-flowing enzyme compositions (C12N9/96 takes precedence) - C12Y 302/01023: Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase - A23G 2200/00: containing organic compounds, e.g. synthetic flavouring agents - A23G 2200/02: containing microorganisms, enzymes, probiotics - A23G: COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF - A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Y: ENZYMES ## Abstract A chemically commercially sterilized enzyme composition for addition to a pasteurized dairy product to reduce gradually the lactose content of the dairy product and to introduce functioning enzymes into the digestive tract of an individual when the dairy product is ingested. ## Claims ### Claim 1 A method for commercially sterilizing an enzyme, comprising the steps of (a) forming a chemical sterilization composition by admixing water, an edible acid, a buffering salt, and an antimicrobial agent selected from the group consisting of sorbic acid, benzoic acid, sodium benzoate, potassium sorbate, sodium sorbate, and potassium benzoate; and, (b) mixing the enzyme with the chemical sterilization composition to form an enzyme sterilization composition, said enzyme sterilization composition having a pH in the range of 2.0 to 4.6 and including (i) 50% to 99% by weight of said water, (ii) 0.1% to 6.0% by weight of said edible acid, (iii) 0.001% to 5.0% by weight of said buffer salt, (iv) 0.01% to 6.0% by weight of said antimicrobial agent, and (v) 0.001% to 45.0% by weight of said enzyme. ### Claim 2 A commercially sterile enzyme powder composition for combination with a dairy product which has been heated to a selected temperature for a time sufficient to at least pasteurize the dairy product, said enzyme composition hydrolyzing lactose to glucose and galactose and consisting of: (a) from 4% to 20% by weight of an edible acid for adjusting the pH of the enzyme composition with the range of about 2.0 to 6.0 when the composition is hydrated; (b) from 2% to 20% by weight of a buffering salt to facilitate maintenance of the pH in the range of 2.0 to 6.0; (c) from 0.02% to 10% by weight of an antimicrobial agent selected from the group consisting of sorbic acid, benzoic acid, sodium benzoate, potassium sorbate, sodium sorbate, and potassium benzoate; and, (d) from 14% to 93% by weight of an enzyme to hydrolyze lactose when the pH is in the range of 2.0 to 6.0. ### Claim 3 (depends on 2) The composition of claim 2 in hydrated form and including from 50% to 99% by weight of water. ### Claim 4 A method for preparing a pasteurized lactose-containing product in which the concentration of lactose is decremented, said method including the steps of (a) providing a dairy product; (b) chemically commercially sterilizing a lactose-hydrolyzing enzyme by admixing (i) from 50% to 99% by weight of water, (ii) from 0.1 to 6% by weight of an edible acid for adjusting the pH of the enzyme composition within the range of about 2.0 to 6.0; (iii) from 0.001% to 5% by weight of a buffer to facilitate maintenance of the pH in the range of 2.0 to 6.0; (iv) from 0.01% to 6% by weight of an antimicrobial agent selected from the group consisting of sorbic acid, benzoic acid, sodium benzoate, potassium sorbate, sodium sorbate, and potassium benzoate; and, (v) from 0.001% to 45% by weight of the lactose-hydrolyzing enzyme to form a commercially sterile enzyme composition; (c) heating the dairy product to a selected temperature for a time sufficient to at least pasteurize the dairy product; (d) combining said chemically sterile enzyme composition with said pasteurized dairy product to produce an enzyme--dairy product combination; (e) packaging said enzyme--dairy product combination; and, (f) storing said packaged enzyme--dairy product combination produced in step (e) to permit said enzyme to hydrolyze lactose in said enzyme--dairy product. ### Claim 5 A method for preparing and storing a pasteurized lactose-containing product in which substantially all of the lactose is hydrolyzed prior to ingestion of the product, said method including the steps of (a) providing a dairy product; (b) chemically commercially sterilizing a lactose-hydrolyzing enzyme by admixing (i) from 50% to 99% by weight of water, (ii) from 0.1 to 6% by weight of an edible acid for adjusting the pH of the enzyme composition within the range of about 2.0 to 6.0, (iii) from 0.001% to 5% by weight of a buffer to facilitate maintenance of the pH in the range of 2.0 to 6.0, (iv) from 0.01% to 6% by weight of an antimicrobial agent selected from the group consisting of sorbic acid, benzoic acid, sodium benzoate, potassium sorbate, sodium sorbate, and potassium benzoate, and, (v) from 0.001% to 45% by weight of the lactose-hydrolyzing enzyme, to form a chemically sterile enzyme composition; (c) heating the dairy product to a selected temperature for a time sufficient to at least pasteurize the dairy product; (d) combining a selected quantity of said chemically sterile enzyme composition with said pasteurized dairy product to produce an enzyme--dairy product combination containing from five parts per million to one hundred and fifty parts per million of said lactose-hydrolyzing enzyme; (e) packaging said enzyme--dairy product combination; and, (f) storing said packaged enzyme--dairy product combination produced in step (e) for a length of time sufficient to permit said enzyme to hydrolyze substantially all lactose in said enzyme--dairy product combination prior to ingestion of said enzyme--dairy product combination by an individual. ### Claim 6 (depends on 5) The method of claim 5 wherein in step (f) said packaged enzyme-dairy product is stored for a period of time in the range of two to seven days. ### Claim 7 A method for preparing, storing, and serving a pasteurized lactose-containing frozen shake product in which substantially all of the lactose is hydrolyzed prior to ingestion of the product, said method including the steps of (a) providing a dairy product; (b) chemically commercially sterilizing a lactose-hydrolyzing enzyme by admixing (i) from 50% to 99% by weight of water, (ii) from 0.1 to 6% by weight of an edible acid for adjusting the pH of the enzyme composition within the range of about 2.0 to 6.0, (iii) from 0.001% to 5% by weight of a buffer to facilitate maintenance of the pH in the range of 2.0 to 6.0, (iv) from 0.01% to 6% by weight of an antimicrobial agent selected from the group consisting of sorbic acid, benzoic acid, sodium benzoate, potassium sorbate, sodium sorbate, and potassium benzoate, and, (v) from 0.001% to 45% by weight of the lactose-hydrolyzing enzyme to form a chemically sterile enzyme composition; (c) heating the dairy product to a selected temperature for a time sufficient to at least pasteurize the dairy product; (d) combining a selected quantity of said chemically sterile enzyme composition with said pasteurized dairy product to produce an enzyme--dairy product combination containing from five parts per million to one hundred and fifty parts per million of said lactose-hydrolyzing enzyme; (e) packaging and freezing said enzyme--dairy product combination such that said lactose-hydrolyzing enzyme continues to hydrolyze lactose after said product combination is frozen; (f) storing said packaged and frozen enzyme--dairy product combination produced in step (e) for a length of time sufficient to permit said enzyme to hydrolyze generally all lactose in said enzyme--dairy product combination prior to ingestion of said enzyme--dairy product combination by an individual; (g) thawing said product combination until it has the consistency of a conventional ice cream milk shake containing ice particles in a liquid matrix; and, (h) serving said thawed product combination produced in step (g) for ingestion by an individual. ### Claim 8 (depends on 7) The method of claim 7 wherein in step (f) said packaged enzyme-dairy product is stored for a period of time in the range of two to fourteen days.