# US 5,707,831 A · Process for preparing recombinant aprotinin and recombinant aprotinin variants having the natural N-terminal sequence

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- Number: US 5,707,831 A
- Document ID: US5707831A
- Status: Granted
- Kind: A
- Application number: 08/439,585
- Filed: 1995-05-11
- Granted: 1998-01-13
- Earliest claimed: 1994-05-18
- Assignees: Bayer Aktiengesellschaft
- Inventors: Heiner Apeler; Jurgen Beunink; Michael Dorschug; Uwe Gottschalk
- Web page: https://patentgrep.com/patent/US5707831A

## CPC

- C07K 14/8117: {Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)}
- C07K 2319/02: containing a signal sequence
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The invention relates to a process for preparing recombinant aprotinin and recombinant aprotinin variants having the natural N-terminal sequence, with the recombinant aprotinin and/or the recombinant aprotinin variants being present as homogeneously processed, secreted proteins.

## Claims

### Claim 1

A vector for expressing in yeast cells aprotinin and aprotinin variants having a correct N terminus, said vector comprising:
a) the gene for the aprotinin or the variant, and
b) as a leader directly upstream of this gene, the nucleotide sequence for the yeast a Mating Factor signal sequence.

### Claim 2 (depends on 1)

A process for preparing aprotinin and aprotinin variants having a correct N terminus, said process comprising:
a) culturing yeast cells expressing the vector according to claim 1; and
b) isolating the aprotinin or aprotinin variant from the culture supernatant.