# US 5,707,824 A · Method of determining the presence or absence of a paraffinophilic microorganism

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- Number: US 5,707,824 A
- Document ID: US5707824A
- Status: Granted
- Kind: A
- Application number: 08/555,734
- Filed: 1995-11-09
- Granted: 1998-01-13
- Earliest claimed: 1995-11-09
- Assignees: Infectech, Inc.
- Inventors: Mitchell S. Felder; Robert A. Ollar
- Web page: https://patentgrep.com/patent/US5707824A

## CPC

- C12Q 1/04: Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)}
- Y10S 435/81: Packaged device or kit
- Y10S 435/863: Mycobacterium
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A method of determining the presence of a paraffinophilic microorganism in a specimen taken from a patient is provided. The method includes providing a receptacle containing an aqueous solution and adjusting the solution to mimic the in vivo clinical conditions of the patient. The method then further includes inoculating the solution with the specimen and then placing in the receptacle a paraffin coated slide to bait the paraffinophilic microorganism. The slide is then analyzed after exposure to the specimen to determine the presence or absence of the paraffinophilic microorganism. An associated apparatus is also disclosed.

## Claims

### Claim 1

A method of determining the presence or absence of a paraffinophilic organism in a specimen taken from a patient having in vivo clinical conditions, said method comprising:
providing a receptacle containing an aqueous solution;
adjusting said aqueous solution to mimic said in vivo clinical conditions of said patient;
inoculating said solution with said specimen;
placing into said receptacle a paraffin coated slide; and
observing said slide after exposure to said specimen to determine the presence or absence of said microorganism.

### Claim 2 (depends on 1)

The method of claim 1, including
said specimen is selected from the group consisting of blood, stomach fluid, urine, cerebral spinal fluid, nasopharyngeal mucosa and saliva.

### Claim 3 (depends on 1)

The method of claim 1, including
determining a pH of said specimen; and
adjusting said aqueous solution to have a pH generally equal to said pH of said specimen.

### Claim 4 (depends on 1)

The method of claim 1, including
employing as said specimen a blood sample of said patient;
determining an electrolyte level of said blood sample; and
adjusting said aqueous solution to have an electrolyte level generally equal to said electrolyte level of said patient's blood.

### Claim 5 (depends on 4)

The method of claim 4, including
adjusting said aqheous solution by adding thereto at least one of the group consisting of sodium, potassium, chloride, magnesium, phosphate and calcium.

### Claim 6 (depends on 1)

The method of claim 1, including
employing said method to determine the presence or absence of at least one member of the group consisting of Micrococcus Paraffinae; Corynebacterium Simplex; Ahnl; Mycococcus (Rhodococcus) Cinnabareus; Ahnl. Mycococcus (Rhodoc) Rhodochrous; Mycobact. Perrugosum Vat. Athanicum; Mycobact. Rubrum Vat. Propanicum; Mycobacterium Hyalinum; Mycobacterium Lacticola; Mycobacterium Album, M. Luteum; Mycobacterium Microti; Mycobacterium Rubrum, Mycobacterium Phlei.; Mycobacterium Phlei, M. Smegmatis; Mycobacterium Testudo; Mycobacterium-Avium-Intracellulare; Nocardia Spp.; Actinomyces; Candida Lipolytica; Candida Tropicalis, Torulopsis Colliculosa; Monila Sp., Hansenula Sp., Torula rossa; Penicillium Sp.; IHNL. Aspergillus Flayus; Aspergillus sp., Penicillium Sp.; Citromyces Sp., Scopulariopsis Sp.; Pseudomonas Fluorescens Liquefaciens; Ahnl, Pem. Fluorescens Denitrificans; Pseudomonas Aeruginosa.