# US 5,707,817 A · Carbohydrate-based vaccine and diagnostic reagent for trichinosis > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,707,817 A - Document ID: US5707817A - Status: Granted - Kind: A - Application number: 08/415,365 - Filed: 1995-03-31 - Granted: 1998-01-13 - Earliest claimed: 1993-02-05 - Assignees: Colorado State University Research Foundation - Inventors: Nancy Wisnewski; Robert B. Grieve; Donald L. Wassom; Michael R. McNeil - Web page: https://patentgrep.com/patent/US5707817A ## CPC - G01N 33/569: for microorganisms, e.g. protozoa, bacteria, viruses - A61K 39/0003: {Invertebrate antigens} - G01N 33/5308: {for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract The present invention relates to Trichinella diagnostic reagents that include a .beta.-tyvelose-containing composition and use of such reagents to detect Trichinella, and particularly Trichinella spiralis infections. The present invention also includes diagnostic kits based on such reagents and therapeutic agents based on the the knowledge that .beta.-tyvelose is produced by Trichinella spiralis parasites. ## Claims ### Claim 1 A diagnostic reagent to detect Trichinella infection comprising .beta.-tyvelose joined through glycosidic linkage to at least one monosaccharide to form an oligosaccharide having at least one .beta.-tyvelose terminal residue. ### Claim 2 (depends on 1) The reagent of claim 1, wherein said reagent is capable of detecting T. spiralis infection. ### Claim 3 (depends on 1) The reagent of claim 1, wherein said .beta.-tyvelose-containing composition is conjugated to a carrier effective to present said composition for reaction with an antibody indicative of infection by a Trichinella parasite. ### Claim 4 (depends on 1) The reagent of claim 1, wherein said monosaccharide is selected from the group consisting of tyvelose, fucose, mannose, N-acetylgalactosamine, and N-acetylglucosamine. ### Claim 5 (depends on 1) The reagent of claim 1, wherein said monosaccharide joined to .beta.-tyvelose comprises N-acetylgalactosamine. ### Claim 6 (depends on 1) The reagent of claim 1, wherein said .beta.-tyvelose-containing composition is selected from the group consisting of a disaccharide, a trisaccharide and a tetrasaccharide. ### Claim 7 (depends on 1) The reagent of claim 1, wherein said composition comprises a racemic mixture of said .beta.-tyvelose-containing composition and an .alpha.-tyvelose-containing composition, said .alpha.-tyvelose-containing composition being selected from the group consisting of .alpha.-tyvelose and .alpha.-tyvelose joined through glycosidic linkage to at least one monosaccharide to form an oligosaccharide having at least one .alpha.-tyvelose terminal residue. ### Claim 8 A method to detect Trichinella infection in an animal comprising: (a) contacting a bodily fluid collected from said animal with a diagnostic reagent comprising a .beta.-tyvelose-containing composition to form an immunocomplex between said diagnostic reagent and an antibody in said bodily fluid indicative of Trichinella infection, said .beta.-tyvelose-containing composition comprising .beta.-tyvelose joined through glycosidic linkage to at least one monosaccharide to form an oligosaccharide having at least one .beta.-tyvelose terminal residue; and (b) determining Trichinella infection by detecting the presence of said immunocomplex formed in step (a), wherein the presence of said immunocomplex indicates Trichinella infection. ### Claim 9 (depends on 8) The method of claim 8, wherein said reagent is capable of detecting T. spiralis infection. ### Claim 10 (depends on 8) The method of claim 8, wherein said .beta.-tyvelose-containing composition is conjugated to a carrier effective to present said composition for reaction with an antibody indicative of infection by a Trichinella parasite. ### Claim 11 (depends on 8) The method of claim 8, wherein said monosaccharide in said .beta.-tyvelose-containing composition is selected from the group consisting of tyvelose, fucose, mannose, N-acetylgalactosamine, and N-acetylglucosamine. ### Claim 12 (depends on 8) The method of claim 8, wherein said monosaccharide joined to .beta.-tyvelose comprises N-acetylgalactosamine. ### Claim 13 (depends on 8) The method of claim 8, wherein said .beta.-tyvelose-containing composition is selected from the group consisting of a disaccharide, a trisaccharide and a tetrasaccharide. ### Claim 14 (depends on 8) The method of claim 8, wherein said composition comprises a racemic mixture of said .beta.-tyvelose-containing composition and an .alpha.-tyvelose-containing composition, said .alpha.-tyvelose-containing composition being selected from the group consisting of .alpha.-tyvelose and .alpha.-tyvelose joined through glycosidic linkage to at least one monosaccharide to form an oligosaccharide having at least one .alpha.-tyvelose terminal residue. ### Claim 15 (depends on 8) The method of claim 8, wherein said animal is selected from the group consisting of pigs, humans and bears. ### Claim 16 (depends on 8) The method of claim 8, wherein said step of determining comprises: (a) contacting said immunocomplex with a labeled compound capable of binding selectively to said immunocomplex or to said antibody within said immunocomplex; and (b) determining the presence of said labeled compound, wherein presence of said labeled compound is indicative of Trichinella infection. ### Claim 17 (depends on 14) The method of claim 14, further comprising a process for discriminating between Trichinella infection and an infection caused by a microorganism selected from the group consisting of Salmonella serogroup D microorganisms, Yersinaia pseudotuberculosis serogroup IV microorganisms and a mixture thereof, in said animal comprising: (a) contacting bodily fluid collected from said animal with an agent comprising at least one fucose-containing composition, said fucose-containing composition being selected from the group consisting of fucose and fucose joined through glycosidic linkage to at least one monosaccharide to form an oligosaccharide, to form an immunocomplex; and (b) determining Trichinella infection by detecting the presence of said immunocomplex formed in step (a), wherein the presence of said immunocomplex indicates Trichinella infection. ### Claim 18 A diagnostic kit for detecting Trichinella infection in an animal, said kit comprising (a) a diagnostic reagent comprising .beta.-tyvelose joined through glycosidic linkage to at least one monosaccharide to form an oligosaccharide having at least one .beta.-tyvelose terminal residue: and (b) a means for detecting Trichinella infection. ### Claim 19 (depends on 18) The kit of claim 18, wherein said reagent is capable of detecting T. spiralis infection. ### Claim 20 (depends on 18) The kit of claim 18, wherein said reagent is immobilized on a substrate. ### Claim 21 (depends on 18) The kit of claim 18, wherein said means of detecting comprises a labeled substance compound capable of binding to an antibody indicative of Trichinella infection. ### Claim 22 (depends on 18) The kit of claim 18, wherein said monosaccharide in said composition is selected from the group consisting of tyvelose, fucose, mannose, N-acetylgalactosamine, and N-acetylglucosamine. ### Claim 23 (depends on 18) The kit of claim 18, wherein said monosaccharide joined to .beta.-tyvelose comprises N-acetylgalactosamine. ### Claim 24 (depends on 18) The kit of claim 18, wherein said composition is selected from the group consisting of a disaccharide, a trisaccharide and a tetrasaccharide. ### Claim 25 (depends on 18) The kit of claim 18, wherein said composition comprises a racemic mixture of said .beta.-tyvelose-containing composition and an .alpha.-tyvelose-containing composition, said .alpha.-tyvelose-containing composition being selected from the group consisting of .alpha.-tyvelose and .alpha.-tyvelose joined through glycosidic linkage to at least one monosaccharide to form an oligosaccharide having at least one .alpha.-tyvelose terminal residue.