# US 5,707,816 A · Immunological cross reactivity between Candida and human tissue or food antigens

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- Number: US 5,707,816 A
- Document ID: US5707816A
- Status: Granted
- Kind: A
- Application number: 08/796,411
- Filed: 1997-02-06
- Granted: 1998-01-13
- Earliest claimed: 1997-02-06
- Assignees: Immunosciences Lab, Inc.
- Inventors: Aristo Vojdani
- Web page: https://patentgrep.com/patent/US5707816A

## CPC

- G01N 33/56961: {Plant cells or fungi}
- G01N 33/5308: {for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites}
- Y10T 436/25: including sample preparation
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

A method of determining immunological cross reactivity between Candida and human tissue or food antigens. Tissue antigen preparations or food extracts are analyzed by electrophoresis. Cross reacting antigens are identified by immunoblotting using anti-Candida antisera.

## Claims

### Claim 1

A method of detecting immunological cross reactivity between a plurality of tissue antigens and Candida albicans or Candida tropicalis antigens in an individual infected with Candida albicans or Candida tropicalis, comprising the steps of:
isolating serum from said individual;
contacting said tissue antigens with said serum; and
determining whether said serum binds one or more of said tissue antigens.

### Claim 2 (depends on 1)

The method of claim 1, wherein said contacting and determining steps comprise enzyme linked immunosorbent assay or immunodiffusion.

### Claim 3 (depends on 1)

The method of claim 1, further comprising separating said tissue antigens by gel electrophoresis and transferring said antigens to a membrane prior to said contacting step.

### Claim 4 (depends on 1)

The method of claim 1, wherein one or more of said tissue antigens has a molecular weight of about 36 kDa or 72 kDa.

### Claim 5

A method of detecting immunological cross reactivity between food antigen and Candida albicans or Candida tropicalis antigens in an individual infected with Candida albicans or Candida tropicalis, comprising the steps of:
isolating serum from said individual;
contacting said food antigens with said serum; and
determining whether said serum binds one or more of said food antigens.

### Claim 6 (depends on 5)

The method of claim 5, wherein said contacting and determining steps comprise enzyme linked immunosorbent assay or immunodiffusion.

### Claim 7 (depends on 5)

The method of claim 5, further comprising separating said tissue antigens by gel electrophoresis and transferring said antigens to a membrane prior to said contacting step.

### Claim 8 (depends on 7)

The method of claim 7, wherein said food antigens have molecular weights between 1 kDa and 250 kDa.

### Claim 9 (depends on 8)

The method of claim 8, wherein said food antigens have molecular weights between about 10 kDa and about 90 kDa.

### Claim 10

A method of determining a diet plan for a patient having a Candida albicans or Candida tropicalis infection, comprising:
isolating serum from said patient;
contacting a plurality of food antigens with said serum; and
determining whether said serum binds one or more of said food antigens, wherein said food antigens which bind said serum are avoided.

### Claim 11 (depends on 10)

The method of claim 10, wherein said contacting and determining steps comprise enzyme linked immunosorbent assay or immunodiffusion.

### Claim 12 (depends on 10)

The method of claim 10, further comprising separating said tissue antigens by gel electrophoresis and transferring said antigens to a membrane prior to said contacting step.

### Claim 13 (depends on 12)

The method of claim 12, wherein said food antigens have molecular weights between 1 kDa and 250 kDa.

### Claim 14 (depends on 13)

The method of claim 13, wherein said food antigens have molecular weights between about 10 kDa and about 90 kDa.