# US 5,707,678 A · Method for microfiltration of milk or colostral whey > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,707,678 A - Document ID: US5707678A - Status: Granted - Kind: A - Application number: 08/630,452 - Filed: 1996-04-11 - Granted: 1998-01-13 - Earliest claimed: 1995-04-12 - Assignees: GalaGen Inc. - Inventors: Antone G. Gregory - Web page: https://patentgrep.com/patent/US5707678A ## CPC - A23J 1/205: {from whey, e.g. lactalbumine} - A23C 9/1425: {by ultrafiltration, microfiltration or diafiltration of whey, e.g. treatment of the UF permeate} - A23C 9/206: {Colostrum; Human milk} - A23J 1/20: from milk, e.g. casein (curds or cheese A23C); from whey - A23J 3/08: Dairy proteins - A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS - A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00) ## Abstract Ultrafiltration of whey containing products at an acid pH reduces calcium levels in the concentrate and provides a concentrate that can be consistently microfiltered. The concentrate can be used, for example, in a method of microfiltering milk, milk serum, colostrum, or colostral serum which provides effective bioburden reduction without substantial loss of immunoglobulins. ## Claims ### Claim 1 A method of preparing a whey produce to be microfiltered, said whey product obtained after removal of fat and casein from milk, milk serum, colostrum or colostral serum comprising adjusting the pH of the whey product to about 4.5 to 5.0 and ultrafiltering the whey product to obtain a whey-containing ultrafilter concentrate, wherein the ultrafilter concentrate can be consistently microfiltered without fouling of a microfilter membrane or depth filter. ### Claim 2 (depends on 1) The method of claim 1 wherein the temperature of the whey product is maintained at about 4.degree. to 50.degree. C. throughout the ultrafiltration process. ### Claim 3 (depends on 1) The method of claim 1 wherein the temperature of the whey product is maintained at about 20.degree. to 40.degree. C. throughout the ultrafiltration process. ### Claim 4 (depends on 1) The method of claim 1 wherein the ultrafilter has a molecular weight cutoff less than or equal to 100,000. ### Claim 5 (depends on 1) The method of claim 1 wherein water is added during the ultrafiltration to diafilter low molecular weight components from the concentrate. ### Claim 6 (depends on 1) The method of claim 1 wherein the concentration of calcium in the ultrafilter concentrate is substantially lower than the concentration of calcium in the whey product. ### Claim 7 (depends on 1) The method of claim 1 wherein the concentration of calcium in the concentrate is about 10 to 100 mg/100 g. ### Claim 8 (depends on 1) The method of claim 1 wherein the concentrate can be consistently microfiltered using a microfilter membrane or a depth filter of about 0.1 to 5.0 microns without fouling of the microfilter membrane. ### Claim 9 A method of preparing a whey product fortified with immunoglobulins comprising the steps of: (a) Collecting milk or milk serum from a mammal; (b) Centrifuging the milk or milk serum to remove fat; (c) Acidifying the remaining defatted milk or milk serum to precipitate casein followed by removal of the casein to give whey; (d) Adjusting the pH of the whey, if necessary, to about 4.5 to 5.0; (e) Ultrafiltering the whey, providing a whey protein concentrate; and (f) microfiltering the concentrate. ### Claim 10 (depends on 9) The method of claim 9 wherein the microfiltration is carried out using a charged depth filter. ### Claim 11 (depends on 9) The method of claim 9 wherein a first microfiltration step is performed between steps (d) and (e). ### Claim 12 (depends on 9) The method of claim 9 wherein the pH of the whey protein concentrate is adjusted to pH 6.0-7.0 prior to microfiltration. ### Claim 13 (depends on 9) The method of claim 9 wherein the mammal is immunized or hyperimmunized before the collection of milk or milk serum. ### Claim 14 (depends on 9) The method of claim 9 wherein the immunoglobulin recovery across the microfiltration step is about 90% or greater. ### Claim 15 (depends on 9) The method of claim 9 wherein the microfiltration of step (f) is performed at a maximum temperature of about 50.degree. C. ### Claim 16 (depends on 9) The method of claim 9 wherein step (e) is performed at a temperature of about 4.degree. to 50.degree. C. ### Claim 17 (depends on 9) The method of claim 9 wherein step (c) is performed at a temperature of about 20.degree. to 40.degree. C. ### Claim 18 (depends on 9) A method according to claim 9 wherein a filter aid is employed in microfiltration step (f). ### Claim 19 (depends on 9) A method according to claim 9 wherein the mammalian milk serum, colostrum or colostral serum is obtained from a mammal selected from the group consisting of cow, goat, sheep, buffalo, water buffalo, llama, rabbit, human, yak and mouse. ### Claim 20 (depends on 9) The method of claim 9 wherein the ultrafilter has a molecular weight cutoff less than or equal to 100,000. ### Claim 21 (depends on 9) The method of claim 9 wherein water is added during the ultrafiltration to diafilter low molecular weight components from the concentrate. ### Claim 22 (depends on 9) The method of claim 9 wherein the concentration of calcium in the concentrate is about 10 to 100 mg/100 g. ### Claim 23 (depends on 9) The method of claim 9 wherein the ultrafilter concentrate can be consistently microfiltered using a microfilter membrane of about 0.1 to 5.0 microns without fouling of the membrane.