# US 5,707,339 A · Method of directly freezing porcine embryos

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- Number: US 5,707,339 A
- Document ID: US5707339A
- Status: Granted
- Kind: A
- Application number: 08/590,760
- Filed: 1996-01-24
- Granted: 1998-01-13
- Earliest claimed: 1995-10-30
- Assignees: The Japanese Research Association for Animal Embryo Transfer Technology
- Inventors: Kenji Kameyama
- Web page: https://patentgrep.com/patent/US5707339A

## CPC

- A61D 19/04: for embryo transplantation
- A01N 1/10: Preservation of living parts
- A01N 1/125: Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
- A61D: VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

A method of directly freezing porcine embryos which includes adding a solution to be frozen including ethylene glycol, propylene glycol, bovine serum and dextran in the presence of basic medium to porcine embryos and freezing the resulting mixture.

## Claims

### Claim 1

A method of freezing a porcine embryo which comprises freezing a porcine embryo in a solution comprising ethylene glycol, propylene glycol, bovine serum, dextran and a basic medium.

### Claim 2 (depends on 1)

A method according to claim 1, wherein the concentration of dextran is about 1% (w/v).

### Claim 3 (depends on 1)

A method according to claim 1, wherein the concentration of bovine serum is about 20% (v/v).

### Claim 4 (depends on 1)

A method according to claim 1, wherein said bovine serum comprises supplemented calf serum.

### Claim 5 (depends on 1)

A method according to claim 1, wherein the concentration of dextran in said solution is about 1% (w/v).

### Claim 6 (depends on 1)

A method according to claim 1, wherein the concentration of bovine serum in said solution is about 20% (v/v).

### Claim 7

A method of propagating a pig which comprises freezing a porcine embryo in a cryoprotectant solution comprising bovine serum, ethylene glycol, propylene glycol, and dextran, thereby forming a frozen porcine embryo, thawing the frozen porcine embryo and transferring the thawed porcine embryo to a recipient animal.

### Claim 8

A method of freezing a porcine embryo which comprises freezing a porcine embryo in a cryoprotectant solution comprising bovine serum, ethylene glycol, propylene glycol, and dextran.

### Claim 9 (depends on 8)

A method according to claim 8, wherein said bovine serum comprises supplemented calf serum.