# US 5,707,149 A · Device and method for measuring the heat of reaction resulting from mixture of a plurality of reagents > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,707,149 A - Document ID: US5707149A - Status: Granted - Kind: A - Application number: 08/534,699 - Filed: 1995-09-27 - Granted: 1998-01-13 - Earliest claimed: 1995-09-27 - Assignees: The Johns Hopkins University - Inventors: Ernesto Freire; George P. Privalov; Peter L. Privalov; Vincent V. Kavina - Web page: https://patentgrep.com/patent/US5707149A ## CPC - G01N 25/4846: {for a motionless, e.g. solid sample} - G01K 17/00: Measuring quantity of heat (measuring temperature by calorimetry G01K3/00 - G01K11/00; specially adapted for measuring thermal properties of materials, e.g. specific heat, heat of combustion G01N) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - G01K: MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR (radiation pyrometry G01J5/00) ## Abstract A method for measuring the heat of reaction resulting from the mixture of a plurality of reagents comprising the steps of providing a first reagent into a compartment; providing a second reagent into the compartment so as to mix the second reagent with the first reagent within the compartment; withdrawing a predetermined amount of the mixed first and second reagents from the compartment; depositing the withdrawn predetermined amount of the mixed first and second reagents back into the compartment; generating electrical signals based on the heat of reaction of the mixed first and second reagents; and deriving data indicative of the heat of reaction based on the electrical signals. ## Claims ### Claim 1 A method for measuring the heat of reaction resulting from the mixture of a plurality of liquid reagents comprising the steps of: providing a first liquid reagent into a compartment; providing a second liquid reagent into said compartment so as to mix said second reagent with said first reagent within said compartment; withdrawing a predetermined amount of the mixed first and second reagents from the compartment; depositing the withdrawn predetermined amount of the mixed first and second reagents back into the compartment; generating electrical signals based on the heat of reaction of the mixed first and second reagents; and deriving data indicative of the heat of reaction based on said electrical signals. ### Claim 2 (depends on 1) The method according to claim 1, further comprising: displacing a portion of the first liquid reagent from said compartment when said second liquid reagent is provided into said compartment. ### Claim 3 (depends on 2) The method according to claim 2, further comprising: utilizing a data processor to receive the electrical signals based on the heat of reaction and to generate output signals proportional to the heat of reaction; utilizing said output signals to maintain said compartment at a generally constant temperature during mixture of said first and second reagents; and wherein said deriving of data indicative of the heat of reaction based on the electrical signals is accomplished by determining a magnitude of the output signals required to maintain the compartment at said generally constant temperature. ### Claim 4 (depends on 2) A method according to claim 2, wherein said compartment has a substantially cylindrical shape, and wherein the depositing of the mixed first and second liquid reagents back into the compartment is done generally tangentially to said cylindrical compartment so as to cause the first and second reagents to circulate in a generally uniform direction within the compartment so as to facilitate complete mixture of said first and second reagents. ### Claim 5 (depends on 1) A method according to claim 1, further comprising repeating the withdrawing and depositing steps at least one additional time to accomplish further stirring of the reagents. ### Claim 6 A method for measuring the heat of reaction resulting from the mixture of a plurality of liquid reagents comprising: filling a compartment with a first liquid reagent; providing a second liquid reagent into said compartment to cause a portion of said first liquid reagent to be displaced from said compartment; withdrawing a predetermined portion of the first and second reagents from the compartment after said portion of the first liquid reagent is displaced from said compartment; depositing the withdrawn predetermined portion of first and second reagents back into the compartment; generating electrical signals based on the heat of reaction resulting from mixture of the first and second reagents; and deriving data indicative of the heat of reaction based on said electrical signals. ### Claim 7 A method for measuring the heat of reaction resulting from the mixture of a plurality of reagents comprising the steps of: initially providing a predetermined amount of first reagent into a compartment; then providing a predetermined amount of second reagent into said compartment to cause said first and second reagents to mix within said compartment; then providing an additional amount of said first reagent into the compartment in such fashion which causes the mixed reagents within the compartment to circulate in a substantially uniform direction within the compartment; generating electrical signals based on the heat of reaction of the mixed reagents within the compartmentp; and deriving data indicative of the heat of reaction based on said electrical signals. ### Claim 8 (depends on 7) A method according to claim 7, wherein the compartment has a substantially circular cross-section through a predetermined plane, and wherein the step of providing an additional amount of said first reagent into the compartment is accomplished by tangentially injecting a stream of said first reagent relative to said circular cross-section so as to cause the mixed reagents within the cell to circulate in said substantially uniform direction within the cell. ### Claim 9 (depends on 7) A method according to claim 7, further comprising the steps of providing an additional amount of said second reagent into the compartment substantially simultaneously with providing the additional amount of said first reagent into the compartment. ### Claim 10 (depends on 7) A method according to claim 7, further comprising the step of calculating the relative concentrations of said first and second reagents within said compartment in order to derive said heat of reaction. ### Claim 11 (depends on 7) A method according to claim 7, further comprising the steps of withdrawing a predetermined portion of the first and second reagents after they are mixed within said compartment and depositing the withdrawn predetermined portion of mixed reagents back into said compartment. ### Claim 12 A device for measuring the heat of reaction resulting from mixture of a plurality of reagents, comprising: a compartment; a first injection assembly for providing a first reagent into said compartment; a second injection assembly for providing a second reagent into said compartment to permit said first and second reagents to initially mix within said compartment, at least one of said first and second injection assemblies being constructed and arranged to be able to i) withdraw a predetermined portion of said first and second reagents from said compartment after the reagents have been initially mixed within said compartment and ii) deposit the withdrawn predetermined portion of said first and second reagents back into the compartment so as to facilitate stirring of said first and second reagents within said compartment; and a measuring apparatus operably connected with said compartment for deriving data indicative of the heat of reaction resulting from mixing said first and second reagents. ### Claim 13 (depends on 12) A device according to claim 12, wherein said compartment is generally cylindrical in shape, and wherein said at least one of the injection assemblies comprises a first injection device and a tangential inlet tube providing a passage between said first injection device and said compartment, said tangential inlet tube being constructed and arranged to direct the deposited predetermined portion of said first and second reagents in a generally circumferential direction within said cylindrical compartment. ### Claim 14 (depends on 13) A device according to claim 13, wherein one of the injection assemblies comprises said first injection device and said tangential inlet tube, and wherein another one of said injection assemblies comprises a second injection device and a radial inlet tube providing a passage between said second injection device and said compartment, said radial inlet tube being constructed and arranged to direct the second reagent towards the center of said compartment when said second reagent is provided into said compartment by said second injection device. ### Claim 15 (depends on 14) A device according to claim 14, further comprising an outlet tube connected with said compartment for permitting a portion of the first reagent to be displaced from the compartment therethrough when said second reagent is provided into said compartment by the first injection device to permit the reagents to initially mix. ### Claim 16 (depends on 12) A device according to claim 12, wherein said measuring apparatus comprises a measuring thermopile connected with said compartment for generating a measuring signal indicative of the heat of reaction resulting from the mixture of said reagents, and a data processor for processing said measuring signal to derive said data indicative of the heat of reaction. ### Claim 17 (depends on 16) A device according to claim 16, wherein said measuring apparatus further comprises a compensating thermopile structurally connected with said compartment and electrically connected with said data processor for receiving compensation signals from said data processor based on said measuring signals and for converting said compensation signals into electric power used to maintain said compartment at a generally constant temperature during the heat of reaction. ### Claim 18 (depends on 12) A device according to claim 12, wherein said injection assemblies comprise automatic syringe injectors electrically connected with said measuring apparatus.