# US 5,705,624 A · DNA sequences encoding enzymes useful in phytoene biosynthesis

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- Number: US 5,705,624 A
- Document ID: US5705624A
- Status: Granted
- Kind: A
- Application number: 08/579,667
- Filed: 1995-12-27
- Granted: 1998-01-06
- Earliest claimed: 1995-12-27
- Inventors: Wayne Paul Fitzmaurice; Gary Mark Hellmann; Laurence Kay Grill; Monto Hiroshi Kumagai; Guy Richard della-Cioppa
- Web page: https://patentgrep.com/patent/US5705624A

## CPC

- C12N 9/00: Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

DNA sequences isolated from Nicotiana species (e.g., Nicotiana tabacum and Nicotiana benthamiana) and encoding polypeptides having enzymatic activity for producing phytoene, and the polypeptides encoded, are provided.

## Claims

### Claim 1

An isolated nucleic acid molecule encoding a polypeptide having enzymatic activity for producing phytoene, said molecule having a sequence selected from the group consisting of:
(a) SEQ ID NO: 1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7 and SEQ ID NO:9;
(b) nucleotide sequences which encode a polypeptide having enzymatic activity for producing phytoene and which hybridize to sequences of (a) above under stringent conditions defined by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, 0.1% SDS at 70.degree. C.;
(c) nucleotide sequences which encode a polypeptide having enzymatic activity for producing phytoene and which differ from sequences of (a) and (b) due to the degeneracy of the genetic code.

### Claim 2

An isolated nucleic acid molecule encoding a polypeptide which has enzymatic activity for producing phytoene, said polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8.

### Claim 3 (depends on 1, 2)

A nucleic acid molecule of claim 1 or 2 in the form of a DNA molecule.

### Claim 4 (depends on 3)

A nucleic acid molecule of claim 3 isolated from a Nicotiana species.