# US 5,705,612 A · NEF peptide encoded by human immunodefiency virus type 1 (HIV-1)

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- Number: US 5,705,612 A
- Document ID: US5705612A
- Status: Granted
- Kind: A
- Application number: 08/195,024
- Filed: 1994-02-14
- Granted: 1998-01-06
- Earliest claimed: 1984-10-18
- Assignees: Institut Pasteur and Centre National de la Recherche Scientifique
- Inventors: Marc Alizon; Pierre Sonigo; Cole Stewart; Oliver Danos; Simon Wain-Hobson
- Web page: https://patentgrep.com/patent/US5705612A

## CPC

- C07K 14/005: from viruses
- A61P 31/18: for HIV
- C12N 2740/16322: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The complete nucleotide sequence of a proviral molecular clone of the lymphadenopathy-associated virus, LAV, was ascertained. Recombinant phage clones were isolated from a genomic library of LAV-infected human T-lymphocyte DNA. The insert of recombinant phase .lambda.J19 was sequenced according to the dideoxy chain termination method. In addition to the retroviral structural genes gag, pol, and env, a novel open reading frame (ORF) was identified in the 3' region of the viral genome and designated ORF-R. The ORF-R coding region, which has subsequently been renamed nef, encodes a 206 amino acid protein that slightly overlaps the 3' end of the env gene and the 5' portion of the U3 region of the viral long terminal repeat (LTR). Nucleic acids encoding this protein and the precise amino acid sequence of the Nef are disclosed in this application. These products are useful for the generation of immunological reagents to facilitate the detection of LAV.

## Claims

### Claim 1

A peptide of HIV-1 expressed from DNA corresponding to the nef open reading frame, the DNA having the following nucleotide sequence: ##STR2##

### Claim 2

A peptide encoded by the nef gene of HIV-1, wherein the peptide is free of particles of said virus and has the following amino acid sequence: ##STR3##