# US 5,705,609 A · Decorin fragments inhibiting cell regulatory factors

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- Number: US 5,705,609 A
- Document ID: US5705609A
- Status: Granted
- Kind: A
- Application number: 08/442,063
- Filed: 1995-05-16
- Granted: 1998-01-06
- Earliest claimed: 1988-06-28
- Assignees: La Jolla Cancer Research Foundation
- Inventors: Erkki I. Ruoslahti; Michael D. Pierschbacher; Jose Cardenas; William Craig; Daniel G. Mullen
- Web page: https://patentgrep.com/patent/US5705609A

## CPC

- C07K 14/4725: {Proteoglycans, e.g. aggreccan}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C07K 2319/00: Fusion polypeptide
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present invention provides a method of inhibiting an activity of a cell regulatory factor comprising contacting the cell regulatory factor with a purified polypeptide, wherein the polypeptide comprises a cell regulatory factor binding domain of a protein. The protein is characterized by a leucine-rich repeat of about 24 amino acids. In a specific embodiment, the present invention relates to the ability of decorin, a 40,000 dalton protein that usually carries a glycosaminoglycan chain, and more specifically to active fragments of decorin or its functional equivalents to bind TGF.beta.. The invention also provides a novel cell regulatory factor designated MRF. Also provided are methods of identifying, detecting and purifying cell regulatory factors and proteins which bind and affect the activity of cell regulatory factors.

## Claims

### Claim 1

A polypeptide comprising the sequence His-Leu-Arg-Val-Val-Gln (SEQ ID NO: 62), wherein the polypeptide is between 6 and 95 amino acids in length.

### Claim 2 (depends on 1)

The polypeptide of claim 1 selected from the group consisting of PT 72 (SEQ ID NO: 31), PT 73 (SEQ ID NO: 34), P.sub.25 -Q.sub.36 (SEQ ID NO: 18), H.sub.31 -S.sub.37 (SEQ ID NO: 17), H.sub.31 -L.sub.42 (SEQ ID NO: 19), and 16G (SEQ ID NO: 24).

### Claim 3 (depends on 1)

The polypeptide of claim 1 having the sequence His-Leu-Arg-Val-Val-Gln-Cys-Ser-Asp-Leu-Gly-Leu (SEQ ID NO: 24).

### Claim 4

A polypeptide whose amino acid sequence is: Ser-Ser-Asp-Phe-Cys-Pro-Pro-Gly-His-Asn-Thr-Lys-Lys-Ala-Ser-Tyr-Ser-Gly-Va l-Ser-Leu-Phe-Ser-Asn-Pro-Val-Gln-Tyr-Trp-Glu-Ile-Gln-Pro-Ser-Thr-Phe-Arg-C ys-Val-Tyr-Val-Arg-Ser-Ala-Ile-Gln-Leu-Gly-Asn-Tyr-Lys (SEQ ID NO: 57, residues 3 to 53).

### Claim 5

A polypeptide whose amino acid sequence is: Ser-Ser-Asp-Phe-Ser-Pro-Pro-Gly-His-Asn-Thr-Lys-Lys-Ala-Ser-Tyr-Ser-Gly-Va l-Ser-Leu-Phe-Ser-Asn-Pro-Val-Gln-Tyr- Trp-Glu-Ile-Gln-Pro-Ser-Thr-Phe-Arg-Cys-Val-Tyr-Val-Arg-Ser-Ala-Ile-Gln-Le u-Gly-Asn-Tyr-Lys (SEQ ID NO: 60, residues 3 to 53).