# US 5,705,486 A · Isolation of novel peptides from green barley leaf extract and use same to inhibit human platelet aggregation

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- Number: US 5,705,486 A
- Document ID: US5705486A
- Status: Granted
- Kind: A
- Application number: 08/615,888
- Filed: 1996-03-14
- Granted: 1998-01-06
- Earliest claimed: 1996-03-14
- Assignees: Yoshihide Hagiwara
- Inventors: Mahnaz Badamchian; Yoshihide Hagiwara; Hideaki Hagiwara
- Web page: https://patentgrep.com/patent/US5705486A

## CPC

- C07K 7/06: having 5 to 11 amino acids
- A61P 7/02: Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Two penta-peptides, Asp-Asp-Asn-Asp-Asn and Asp-Asp-Ser-Gln-Gln, extracted from green barley leaves, have been found to inhibit the adenosine diphosphate (ADP)-induced aggregation of human blood platelets.

## Claims

### Claim 1

A composition consisting of the penta-peptide Asp-Asp-Asn-Asp-Asn.

### Claim 2

A composition consisting of the penta-peptide Asp-Asp-Ser-Gln-Gln.

### Claim 3 (depends on 1)

A composition for the inhibition of blood platelet aggregation consisting essentially of a blood platelet aggregation inhibitory effective amount of the composition according to claim 1 and a pharmaceutically acceptable carrier therefor.

### Claim 4 (depends on 2)

A composition for the inhibition of blood platelet aggregation consisting essentially of a blood platelet aggregation inhibitory effective amount of the composition according to claim 2 and a pharmaceutically acceptable carrier therefor.

### Claim 5

A method for inhibiting blood platelet aggregation in a mammal comprising administering a blood platelet aggregation inhibitory effective amount of the penta-peptide Asp-Asp-Asn-Asp-Asn to said mammal.

### Claim 6

A method for inhibiting blood platelet aggregation in a mammal comprising administering a blood platelet aggregation inhibitory effective amount of the penta-peptide Asp-Asp-Ser-Gln-Gln to said mammal.