Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets
AssigneesIGEN International, Inc., Eisai Co., Ltd.
InventorsJonathan K. Leland, Haresh P. Shah, John H. Kenten, Jack E. Goodman, George E. Lowke, Yuzaburo Namba, Gary F. Blackburn, Richard J. Massey
Life of this patent
Estimating the term…
Abstract
Disclosed and claimed are methods and apparatus for performing a binding assay for an analyte of interest present in a sample based upon measurement of electrochemiluminescence at an electrode. The method uses magnetically responsive particles. The method and apparatus call for a plurality of electromagnets or permanent magnets in north-south orientation for imposing a magnetic field so as to collect the particles.
Claims8
A method for performing a binding assay for, and detecting or qauantitating, an analyte of interest in a sample comprising the steps of:
- (a) forming a composition comprising:
- (i) said sample:
- (ii) an assay-performance-substance comprising an electrochemiluminescent label and containing at least one component selected from the group consisting of:
- (1) added analyte of interest or added analogue of said analyte;
- (2) a binding partner of said analyte or said analogue;
- (3) a reactive component capable of binding with component (1) or (2);
- and
- (iii) a plurality of magnetically responsive suspended particles to which are bound a binding partner of said analyte and/or said assay-performance-substance;
- (b) incubating said composition to form a complex which includes a particle and said electrochemiluminescent label;
- (c) introducing said composition into an assay cell;
- (d) collecting said complex at the surface of an electrode by imposition of a magnetic field on said particles, said magnetic field being imposed by a plurality of magnets in north-south orientation;
- (e) inducing the electrochemiluminescent label compound in said complex to electrochemiluminesce by imposing a voltage on said electrode;
- and
- (f) detecting or quantifying the electrochemiluminescence emitted at the electrode surface to detect or quantify the presence of the analyte of interest in the sample.
Dependencies are inferred from the claim text.