# US 5,705,397 A · Analytical devices and methods of use > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,705,397 A - Document ID: US5705397A - Status: Granted - Kind: A - Application number: 08/495,539 - Filed: 1995-08-25 - Granted: 1998-01-06 - Earliest claimed: 1993-03-04 - Assignees: British Technology Group Limited - Inventors: Roger Abraham Bunce - Web page: https://patentgrep.com/patent/US5705397A ## CPC - B01L 3/5023: {with a sample being transported to, and subsequently stored in an absorbent for analysis} - G01N 33/5302: {Apparatus specially adapted for immunological test procedures} - B01L 2200/12: Specific details about manufacturing devices - B01L 2300/0825: Test strips - B01L 2300/087: Multiple sequential chambers - B01L 2400/0406: capillary forces - B01L 2400/084: Passive control of flow resistance - Y10T 436/2575: Volumetric liquid transfer - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract An analytical device (1) for use in assay procedures comprises at least one liquid flow channel of porous material leading from a channel end to an analytical site (2) via a localized reagent site (3). A liquid-impermeable barrier (6) is arranged adjacent to the reagent site (3) in the liquid flowpath to slow the transport of the reagent to the analytical site (2) by creating a substantially stagnant zone (10) which has the effect of elongating the distribution of reagent in the liquid flow. ## Claims ### Claim 1 An analytical device for use in assay procedures, comprising at least one liquid flow channel of porous material for capillary liquid flow therealong, the channel having: a channel end for liquid application thereto, an analytical site, said channel defining a liquid flow path leading in a downstream direction from said channel end to said analytical site, a localized reagent site said channel end and said analytical site, and a permanent liquid impermeable barrier in said channel between said channel end and said analytical site, said barrier being constructed and adapted to create a substantially stagnant zone by intercepting said liquid flow path and extending partially across the channel width, sufficiently close to said localized reagent site that, on application of a reagent to said localized reagent site and application of liquid to said channel end, said substantially stagnant zone is created whereby the distribution of said reagent is elongated as it is delivered from said reagent site to said analytical site by entrainment in said capillary flow. ### Claim 2 (depends on 1) An analytical device according to claim 1, wherein said liquid-impermeable barrier is provided by application of an agent to render the porous material impermeable in a selected region. ### Claim 3 (depends on 1) An analytical device according to claim 1, wherein said liquid-impermeable barrier is provided by absence of porous material in a selected region. ### Claim 4 (depends on 1) An analytical device according to claim 1, wherein said liquid-impermeable barrier is on the upstream side of the reagent site. ### Claim 5 (depends on 1) An analytical device according to claim 1, wherein said liquid-impermeable barrier is on the downstream side of the reagent site. ### Claim 6 (depends on 1) An analytical device according to claim 1, wherein said liquid-impermeable barrier is shaped as a rectangular bar arranged substantially perpendicularly across the liquid flow path. ### Claim 7 (depends on 1) an analytical device according to claim 1, wherein said liquid impermeable barrier is substantially V-shaped. ### Claim 8 (depends on 7) An analytical device according to claim 7, wherein the arms of the V-shaped liquid-impermeable barrier extend on either side of the reagent site in a downstream direction. ### Claim 9 (depends on 1) An analytical device according to claim 1, wherein said liquid impermeable barrier features two V-shaped recesses on its downstream side. ### Claim 10 (depends on 9) An analytical device according to claim 9, wherein a different reagent is placed within each of the V-shaped recesses. ### Claim 11 (depends on 1) An analytical device according to claim 1, wherein said liquid impermeable barrier includes two sides which converge to meet at a point at its downstream end. ### Claim 12 (depends on 1) An analytical device according to claim 1, wherein said liquid flow channel features a variation in channel width in the region of the reagent site. ### Claim 13 (depends on 12) An analytical device according to claim 12, wherein the variation is a narrowed portion in the channel. ### Claim 14 A method of controlling the rate of delivery of a reagent to an analytical site in an assay procedure, said method comprising: providing at least one porous liquid flow channel in which an analytical site is located, said channel also having a channel end for liquid application thereto, and incorporating a localized reagent site, said channel also having a permanent liquid impermeable barrier between the channel end and said analytical site, said barrier extending partially across the width of said channel; providing a selected reagent at the reagent site; introducing liquid to said channel end to initiate capillary liquid flow along the flow channel to the analytical site via the reagent site, the impermeable barrier creating a substantially stagnant zone, said impermeable barrier being sufficiently close to the reagent site such that the substantially stagnant zone results in a slowing of transport of the reagent relative to transport of said liquid as it is delivered to the analytical site by entrainment in said capillary flow. ### Claim 15 (depends on 14) A method according to claim 14, whereby the form of the relatively stagnant zone is controlled by selecting the flow characteristics within the flow channel. ### Claim 16 (depends on 15) A method according to claim 15, whereby selected flow characteristics are determined by varying the width of the flow channel. ### Claim 17 An analytical device for use in assay procedures, comprising at least one liquid flow channel of porous material for capillary liquid flow therealong, the channel having: a channel end for liquid application thereto, an analytical site, the channel defining a liquid flow path leading in a downstream direction from said channel end to said analytical site, a localized reagent site in said liquid flow path between said channel end and said analytical site, and permanent liquid impermeable barrier means between said channel end and said analytical site and extending partially across the channel width for intercepting said liquid flow path on application of a reagent to said localized reagent site and application of liquid to said channel end, said barrier means being constructed and adapted to create a substantially stagnant zone, whereby transport of said reagent from said reagent site to said analytical site is slowed relative to transport of said liquid.