Method for diagnosis of thrombotic disorders
AssigneesThe Scripps Research Institute, The Regents of the University of California
InventorsJohn H. Griffin, Samuel I. Rapaport, Dzung T. Le
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Abstract
The present invention provides an in vitro method useful for the diagnosis of a thrombotic disorder in a subject, having or at risk of having the disorder. Specifically, the disorder exemplified herein is associated with APC resistant Factor V and Va. The clotting time of a test sample is analyzed in the presence and absence of APC and compared with a standard reference sample in order to diagnose the subject.
Claims18
An in vitro method for diagnosing a subject as having or as being at risk for having a thrombotic disorder associated with activated protein C (APC)-resistant factor V or Va, the method comprising:
- a. contacting a first test sample comprising a coagulation factor V or Va-containing specimen from the subject with a procoagulant reagent, factor V-deficient plasma to provide coagulation factors other than factors V or Va, calcium sufficient to initiate clotting, and APC in a first test reaction;
- b. contacting a second test sample comprising a coagulation factor V or Va-containing specimen from the subject with a procoagulant reagent, factor V-deficient plasma to provide coagulation factors other than factors V or Va, and calcium sufficient to initiate clotting in a second test reaction;
- and
- c. comparing the clotting times for the first and second test reactions, wherein detection of similar clotting times in the first and second test reactions indicates a diagnosis of a thrombotic disorder associated with APC-resistant factor V or Va.
Dependencies are inferred from the claim text.