# US 5,705,386 A · Circular plasmids

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- Number: US 5,705,386 A
- Document ID: US5705386A
- Status: Granted
- Kind: A
- Application number: 08/678,650
- Filed: 1996-07-11
- Granted: 1998-01-06
- Earliest claimed: 1995-07-20
- Assignees: Nitto Chemical Industry Co., Ltd.
- Inventors: Wataru Mizunashi; Fujio Yu
- Web page: https://patentgrep.com/patent/US5705386A

## CPC

- C12N 15/74: Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The specification relates to circular plasmids derived from microorganisms of the genus Rhodococcus, which have sizes of about 3.6 kb and restriction site numbers of BamHI:1, BglII:1, ClaI:1, PstI:1, PvuII:2 and SacI:1. The circular plasmids of the present invention are useful as vectors in industrially useful host-vector systems.

## Claims

### Claim 1

An isolated circular plasmid obtained from a microorganism of the genus Rhodococcus; which has a size of about 3.5 kb, wherein the restriction sites have the following order: BglII, PvuII, PstI, PvuII, BamHI, SacI and ClaI.

### Claim 2 (depends on 1)

The isolated circular plasmid of claim 1, wherein the microorganism is Rhodococcus erythropolis ATCC 21035.

### Claim 3 (depends on 2)

The isolated circular plasmid of claim 2, wherein said plasmid is pRC020 .