# US 5,705,378 A · Maltose phosphorylase, trehalose phosphorylase, novel strain of genus plesiomonas capable of producing these enzymes and process for producing trehalose

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- Number: US 5,705,378 A
- Document ID: US5705378A
- Status: Granted
- Kind: A
- Application number: 08/528,923
- Filed: 1995-09-15
- Granted: 1998-01-06
- Earliest claimed: 1994-09-16
- Assignees: Nihon Shokuhin Kako Co., Ltd.
- Inventors: Masahiro Yoshida; Nobuyuki Nakamura; Koki Horikoshi
- Web page: https://patentgrep.com/patent/US5705378A

## CPC

- C12N 9/1051: {Hexosyltransferases (2.4.1)}
- C12N 1/205: Bacterial isolates
- C12P 19/12: Disaccharides
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Disclosed are a novel microorganism (FERM BP-5144) belonging to the genus Plesiomonas and having ability to produce maltose phosphorylase and trehalose phosphorylase required for the enzymatic production of trehalose and novel maltose phosphorylase and trehalose phosphorylase obtainable from the microorganism as well as a process for producing the enzymes. A novel process for enzymatically producing trehalose (O-.alpha.-D-glucopyranosyl-(1.fwdarw.1)-D-glucopyranoside) is also disclosed.

## Claims

### Claim 1

A biologically pure culture of a microorganism having all the identifying characteristics of FERM BP-5144, having an ability to produce maltose phosphorylase and trehalose phosphorylase, and belonging to the genus Plesiomonas.

### Claim 2 (depends on 1)

A process for producing maltose phosphorylase and/or trehalose phosphorylase comprising culturing the microorganism of claim 1, and recovering either or both of the enzymes.

### Claim 3 (depends on 2)

A process of claim 2 wherein the microorganism is cultured in the presence of maltose as a carbon source.

### Claim 4 (depends on 2)

A process of claim 2 wherein the microorganism is cultured in the presence of trehalose as a carbon source.

### Claim 5 (depends on 2)

A process of claim 2 wherein the cultured microbial cells are separated from the culture medium.

### Claim 6 (depends on 2)

A process of claim 2 wherein the cultured microbial cells are separated from the culture medium and maltose phosphorylase and/or trehalose phosphorylase are extracted from the separated cells.

### Claim 7 (depends on 2)

A process of claim 2 wherein the cultured microbial cells are separated from the culture medium and culture supernatant is reserved.