# US 5,705,377 A · Tyrosine kinase

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- Number: US 5,705,377 A
- Document ID: US5705377A
- Status: Granted
- Kind: A
- Application number: 08/447,408
- Filed: 1995-05-23
- Granted: 1998-01-06
- Earliest claimed: 1991-07-03
- Assignees: The Salk Institute for Biological Studies
- Inventors: Merl F. Hoekstra
- Web page: https://patentgrep.com/patent/US5705377A

## CPC

- C12N 9/1205: {Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases}
- C07K 16/40: against enzymes
- C12Q 1/68: involving nucleic acids
- G01N 33/5008: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics}
- G01N 33/5014: {for testing toxicity}
- G01N 33/68: involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- G01N 2500/00: Screening for compounds of potential therapeutic value
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Tyrosine kinase mutant and wild-type genes useful in screening compositions which may affect DNA double-strand break repair activity.

## Claims

### Claim 1

An isolated polynucleotide encoding the S. cerevisiae HRR25 protein kinase polypeptide of Sequence I.D. No. 2.

### Claim 2

An isolated polynucleotide encoding the polypeptide of Sequence I.D. No. 2 wherein an aspartic acid residue replaces the glycine residue at position 151.

### Claim 3

An isolated polynucleotide encoding only a polypeptide having greater than 35 percent amino acid sequence homology to the protein kinase domain amino acid residues 1-287 of SEQ ID NO: 2, wherein said polypeptide:
a) possesses protein kinase activity;
b) promotes normal mitotic recombination; and
c) promotes repair of a DNA strand break occurring at an HO endonuclease site.

### Claim 4 (depends on 1, 2, 3)

A biologically functional plasmid or viral DNA vector comprising a DNA polypeptide according to claim 1, 2 or 3.

### Claim 5 (depends on 1, 2)

A host cell transformed or transfected with a DNA polynucleotide according to claim 1, 2, or 3.

### Claim 6 (depends on 5)

A process for recombinant production of a protein kinase polypeptide comprising growing host cells according to claim 5 under nutrient conditions suitable to allow expression of the protein kinase therein.