US 5,705,364 A · Mammalian cell culture process

Abstract

The present invention relates to novel process for the preparation of glycoproteins by mammalian cell culture wherein the sialic acid content of the glycoprotein produced is controlled over a broad range of values by manipulating the cell culture environment. The invention provides for processes in which the sialic acid content of the glycoprotein is modified by changes in cell culture parameters which affect cell specific productivity. Preferred embodiments of the invention include cell culture processes in the osmolality of the cell culture is controlled as well as the concentration of a transcription enhancer during the production phase of the cell culture. The invention further provides for novel preparations of soluble type 1 tumor necrosis factor immunoglobulin G1 and their uses in the treatment of inflammatory or immune related disorders.

Claims21

Dependent claims

A process for controlling the amount of sialic acid present on an oligosaccharide side chain of a glycoprotein produced by culture in a mammalian host cell which comprises:

culturing the mammalian host cell in a production phase of the culture which is characterized by:
i) adding an alkanoic acid or a salt thereof to the culture at a concentration of about 0.1 mM to about 20 mM;
ii) maintaining the osmolality of the culture at about 250 to about 600 mOsm;
and
iii) maintaining the temperature of the culture at a temperature about between 30.degree. C. and 35.degree. C.

2 more elements

Dependencies are inferred from the claim text.

Mammalian cell culture process

Life of this patent

Mammalian cell culture process

Life of this patent

Abstract

Claims21