# US 5,705,359 A · Expression of heterologous proteins in drosophila cells

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- Number: US 5,705,359 A
- Document ID: US5705359A
- Status: Granted
- Kind: A
- Application number: 08/434,095
- Filed: 1995-05-03
- Granted: 1998-01-06
- Earliest claimed: 1987-05-08
- Assignees: SmithKline Beecham Corporation
- Inventors: Hanne Ranch Johansen; Ariane Adrienne Van Der Straten-Ponthoz; Martin Rosenberg
- Web page: https://patentgrep.com/patent/US5705359A

## CPC

- C07K 14/005: from viruses
- C07K 16/1145: {Env proteins, e.g. gp41, gp110/120, gp160, V3, principal neutralising domain [PND] or CD4-binding site}
- C12N 9/6459: {t-plasminogen activator (3.4.21.68), i.e. tPA}
- C12N 15/85: for animal cells
- C12Y 304/21069: Protein C activated (3.4.21.69)
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- C12N 2740/16122: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2800/90: Vectors containing a transposable element
- C12N 2830/002: inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
- C12N 2830/75: from invertebrates
- C12N 2830/80: from vertebrates
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present invention provides a method for the expression of heterologous genes, under the control of a Drosophila metollothionein promoter, inserted at high copy number into Drosophila melanogaster cells.

## Claims

### Claim 1

A method for regulated expression of heterologous genes inserted at high copy number into cultured Drosophila melanogaster cells which comprises:
transfecting Drosophila melanogaster cells with a gene expression unit having a Drosophila metallothionein promoter and a DNA sequence encoding a heterologous gene product;
culturing the transfected cells in a suitable culture medium; and
inducing said metallothionein promoter by the addition of copper or cadmium to the culture medium such that the heterologous gene product is expressed.

### Claim 2 (depends on 1)

The method of claim 1 wherein the cadmium is present in the culture medium at a final concentration of 10 .mu.M.

### Claim 3 (depends on 1)

The method of claim 1 wherein the copper is present in the culture medium at a final concentration of 500 .mu.M.