# US 5,705,352 A · Multiple drug resistance gene of Aspergillus fumigatus

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 5,705,352 A
- Document ID: US5705352A
- Status: Granted
- Kind: A
- Application number: 08/394,880
- Filed: 1995-02-27
- Granted: 1998-01-06
- Earliest claimed: 1995-02-27
- Assignees: Eli Lilly and Company
- Inventors: Robert B. Peery; Paul L. Skatrud
- Web page: https://patentgrep.com/patent/US5705352A

## CPC

- C07K 14/38: from Aspergillus
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The invention provides isolated nucleic acid compounds encoding a multiple drug resistance protein of Aspergillus fumigatus. Vectors and transformed host cells comprising the multiple drug resistance-encoding DNA of Aspergillus fumigatus MDR-1 are also provided. The invention further provides assays which utilize these transformed host cells.

## Claims

### Claim 1

An isolated nucleic acid molecule that comprises nucleic acid encoding Aspergillus fumigatus MDR-1.

### Claim 2 (depends on 1)

A nucleic acid molecule of claim 1 which is DNA.

### Claim 3 (depends on 2)

The nucleic acid molecule of claim 2 that is SEQ. ID. No. 1.

### Claim 4 (depends on 1)

The nucleic acid molecule of claim 1 that is genomic DNA.

### Claim 5 (depends on 1)

A nucleic acid molecule of claim 1, which is labeled with a detectable moiety.

### Claim 6 (depends on 5)

A nucleic acid molecule of claim 5 wherein the detectable moiety is selected from the group consisting of a fluorescent label, a radioactive atom, and a chemiluminescent label.

### Claim 7 (depends on 1)

A vector comprising a nucleic acid of claim 1.

### Claim 8 (depends on 2)

A vector comprising a nucleic acid of claim 2.

### Claim 9 (depends on 3)

A vector comprising a nucleic acid of claim 3.

### Claim 10 (depends on 4)

A vector comprising a nucleic acid of claim 4.

### Claim 11 (depends on 7)

A host cell containing a vector of claim 7.

### Claim 12 (depends on 8)

A host cell containing a vector of claim 8.

### Claim 13 (depends on 9)

A host cell containing a vector of claim 9.

### Claim 14 (depends on 10)

A host cell containing a vector of claim 10.

### Claim 15 (depends on 11)

A host cell of claim 11 that is a yeast cell.

### Claim 16 (depends on 12)

A host cell of claim 12 that is a yeast cell.

### Claim 17 (depends on 13)

A host cell of claim 13 that is a yeast cell.

### Claim 18 (depends on 14)

The host cell of claim 14 that is a yeast cell.

### Claim 19

A method for determining the fungal MDR inhibition activity of a compound which comprises:
a) growing a culture of yeast cells, transformed with a vector which provides expression of the Afu-MDR-1, in the presence of:
(i) an antifungal agent to which said yeast cell is resistant, but to which said yeast cell is sensitive in its untransformed state;
(ii) a compound suspected of possessing Aspergillus fumigatus MDR inhibition activity; and
b) determining the fungal MDR inhibition activity of said compound by measuring the ability of the antifungal agent to inhibit the growth of said yeast cell.

### Claim 20 (depends on 19)

A method of claim 19 wherein the yeast cell is Saccharomyces cerevisiae .