# US 5,705,341 A · MDC proteins and DNAs encoding the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,705,341 A - Document ID: US5705341A - Status: Granted - Kind: A - Application number: 08/484,355 - Filed: 1995-06-07 - Granted: 1998-01-06 - Earliest claimed: 1993-05-14 - Assignees: Cancer Institute; Eisai Co., Ltd. - Inventors: Yusuke Nakamura; Mitsuru Emi - Web page: https://patentgrep.com/patent/US5705341A ## CPC - C07K 14/4703: {Inhibitors; Suppressors} - C07K 16/18: against material from animals or humans - C07K 2319/00: Fusion polypeptide - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract The present invention provide a gene present in a commonly deleted region of a chromosome in breast and ovarian cancers and encoding a novel protein, the protein ("MDC protein") encoded by the gene and a method for the diagnosis of cancer by using an antibody combinable to the protein. A detailed genetic map of human chromosome 17 was constructed to analyze the chromosome in breast and ovarian cancer tissues, and a gene encoding a novel protein was cloned and its structure was determined. As a result of gene analysis using DNA probes derived from the gene, a gene mutation was confirmed in breast cancer tissues. Moreover, a transformant carrying a plasmid containing the gene was grown to obtain the MDC protein. Furthermore, a monoclonal antibody was prepared by using the protein as an antigen. ## Claims ### Claim 1 A DNA encoding an MDC protein, said DNA being represented by a sequence selected from the group consisting of SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8 and SEQ ID NO:9. ### Claim 2 (depends on 1) The DNA as claimed in claim 1, which comprises the DNA represented by SEQ ID NO:6. ### Claim 3 (depends on 1) The DNA as claimed in claim 1, which comprises the DNA represented by SEQ ID NO:7. ### Claim 4 (depends on 1) The DNA as claimed in claim 1, which comprises the DNA represented by SEQ ID NO:8. ### Claim 5 (depends on 1) The DNA as claimed in claim 1, which comprises the DNA represented by SEQ ID NO:9, including exons and introns therein. ### Claim 6 (depends on 1) A vector containing the DNA as claimed in claim 1. ### Claim 7 (depends on 5) A vector containing the DNA as claimed in claim 5. ### Claim 8 (depends on 6) A transformant carrying the vector as claimed in claim 6. ### Claim 9 (depends on 7) A transformant carrying the vector as claimed in claim 7. ### Claim 10 A DNA encoding an MDC protein, said DNA being represented by at least 13 consecutive bases of a sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8 and SEQ ID NO:9. ### Claim 11 (depends on 10) The DNA as claimed in claim 10, in which the sequence is SEQ ID NO:9, including exons and introns therein. ### Claim 12 (depends on 10) A primer or probe which has a DNA sequence comprising at least 13 consecutive bases of the DNA as claimed in claim 10, or a DNA sequence complementary to the at least 13 consecutive bases of the DNA as claimed in claim 10. ### Claim 13 (depends on 11) A primer or probe which has a DNA sequence comprising at least 13 consecutive bases of the DNA as claimed in claim 11, or a DNA sequence complementary to the at least 13 consecutive bases of the DNA as claimed in claim 11. ### Claim 14 (depends on 12) The primer or probe as claimed in claim 12, wherein the DNA sequence comprises from 15-25 consecutive bases. ### Claim 15 (depends on 13) The primer or probe as claimed in claim 13, wherein the DNA sequence comprises from 15-25 consecutive bases. ### Claim 16 (depends on 12) A gene analysis method which comprises the step of hybridizing the primer or probe as claimed in claim 12 to a DNA to be tested. ### Claim 17 (depends on 13) A gene analysis method which comprises the step of hybridizing the primer or probe as claimed in claim 13 to a DNA to be tested.