# US 5,705,205 A · Process for the production of natural vanilla extract by enzymatic processing of green vanilla pods, and extract thereby obtained

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- Number: US 5,705,205 A
- Document ID: US5705205A
- Status: Granted
- Kind: A
- Application number: 08/714,513
- Filed: 1996-09-16
- Granted: 1998-01-06
- Earliest claimed: 1991-09-03
- Assignees: Pernod Richard
- Inventors: Pascal Marc Brunerie
- Web page: https://patentgrep.com/patent/US5705205A

## CPC

- C12P 7/42: Hydroxy-carboxylic acids
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

A process for increasing the yield of natural vanilla flavor. Green vanilla pods are hydrated. The resulting hydrated pods are ground, forming a liquid phase and a solid phase. The resulting ground hydrated product of green vanilla pods is treated with an enzymatic system including at least one enzyme. The enzyme system possesses from about 10 to about 1000 units of beta-glucose activity per gram of green vanilla pods. The ground hydrated green vanilla pods and the enzymatic system are incubated at a temperature of from about 10.degree. C. to about 40.degree. C. for a period of between about 2 hours and about 30 hours sufficient to allow the release of the vanilla flavor. The liquid phase containing the vanilla flavor is separated from the solid phase.

## Claims

### Claim 1

A process for preparing vanillin from vanilla beans which comprises:
a) hydrating green vanilla pods;
b) grinding the resulting hydrated pods to form a ground hydrated product having a liquid phase and a solid phase;
c) treating said ground hydrated product with an enzymatic system which comprises: (a) at least one enzyme capable of destroying the cell membrane systems of plant cells and wherein said enzyme is selected from the group consisting of an enzyme having pectinase activity, an enzyme having cellulase activity, an enzyme having hemicellulase activity and mixtures thereof and (b) at least one enzyme possessing from about 10 to about 1000 units of a beta-glucosidase activity per gram of green vanilla pods;
d) incubating said ground hydrated product containing said enzymatic system at conditions of a temperature of from about 10.degree. C. to about 40.degree. C. for a period of from about 2 hours to about 30 hours, said conditions being sufficient to enzymatically breakdown cell membranes of said ground hydrated product and allow the release of the vanillin;
e) separating the liquid phase from the solid phase and;
f) recovering said liquid phase which contains the vanillin and other soluble material.

### Claim 2 (depends on 1)

The process as claimed in claim 1 wherein the ground hydrated product has a pH between 3 and 7.

### Claim 3 (depends on 1)

The process as claimed in claim 1, wherein the period of incubation of the mixture of ground product and the enzymatic system is between 3 and 30 hours.

### Claim 4 (depends on 1)

A process according to claim 1; further comprising:
g) isolating the vanilla flavor from the liquid phase.

### Claim 5 (depends on 1)

A process according to claim 1, wherein the at least one enzyme (a) is a cellulase.

### Claim 6 (depends on 2)

A process according to claim 2, wherein the pH of the liquid phase is about 5.

### Claim 7 (depends on 1)

A process according to claim 1, wherein the at least one enzyme (b) possesses 20 to 500 units of beta-glucosidase activity per gram of green vanilla pods.

### Claim 8 (depends on 1)

A process according to claim 1, wherein the at least one enzyme (b) possesses 40 to 400 units of beta-glucosidase activity per gram of green vanilla pods.

### Claim 9 (depends on 1)

A process according to claim 1, wherein said ground hydrated product and said enzymatic system are incubated at a temperature of from about 30.degree. C. to about 40.degree. C.

### Claim 10 (depends on 5)

The process as claimed in claim 5, wherein the at least one enzyme (b) possesses 20 to 500 units of beta-glucosidase activity per gram of green vanilla pods.

### Claim 11 (depends on 5)

The process as claimed in claim 5, wherein the ground hydrated product has a pH of the liquid phase is between 3 and 7.

### Claim 12 (depends on 5)

The process as claimed in claim 5, wherein the period of incubation of the mixture of ground product and the enzymatic system is between 3 and 30 hours.