# US 5,703,219 A · Nucleic acid encoding helicobacter pylori enolase > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,703,219 A - Document ID: US5703219A - Status: Granted - Kind: A - Application number: 08/446,920 - Filed: 1995-05-22 - Granted: 1997-12-30 - Earliest claimed: 1994-03-21 - Assignees: Vanderbilt University - Inventors: Stuart A. Thompson; Martin J. Blaser - Web page: https://patentgrep.com/patent/US5703219A ## CPC - C12N 9/88: Lyases (4.) - C07K 14/205: from Campylobacter (G) - C12N 9/00: Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00) - A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53) - Y10S 435/822: using bacteria or actinomycetales - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS ## Abstract An isolated nucleic acid encoding the Helicobacter pylori recombinase is provided. Also provided is an isolated nucleic acid that hybridizes with the recombinase-encoding nucleic acid under stringent conditions and has at least 70% complementarity with the segment of the nucleic acid of SEQ ID NO:1 to which it hybridizes. Also provided is a mutant strain of H. pylori that does not express a functional recombinase (recA.sup.- mutant). An isolated nucleic acid encoding the Helicobacter pylori enolase is provided. Also provided is an isolated nucleic acid that hybridizes with the nucleic acid that encodes a H. pylori recombinase under stringent conditions and has at least 70% complementarity with the segment of the nucleic acid of SEQ ID NO:9 to which it hybridizes. Also provided is a mutant strain of H. pylori that does not express a functional enolase (eno.sup.- mutant). A method is provided for immunizing a subject against infection by H. pylori by administering to the subject an immunogenic amount of mutant H. pylori in a carrier for the mutant. ## Claims ### Claim 1 An isolated nucleic acid encoding a Helicobacter pylori enolase. ### Claim 2 (depends on 1) The nucleic acid of claim 1 in a vector suitable for expressing the nucleic acid. ### Claim 3 (depends on 2) The nucleic acid of claim 2 in a host cell suitable for expressing the nucleic acid encoding the enolase. ### Claim 4 (depends on 1) The nucleic acid of claim 1, wherein the nucleic acid encodes amino acids 1 through 177 of the amino acid sequence defined in the Sequence Listing as SEQ ID NO:11. ### Claim 5 (depends on 1) The nucleic acid of claim 1, comprising a mutation that results in a non-functional enolase. ### Claim 6 (depends on 4) An isolated nucleic acid of at least 35 nucleotides that hybridizes with the nucleic acid of claim 4 under stringent conditions and has at least 85% complementarity with the segment of the nucleic acid to which it hybridizes, wherein the hybridizing nucleic acid encodes a Helicobacter pylori enolase or a portion thereof. ### Claim 7 (depends on 6) The nucleic acid of claim 6 in a vector suitable for expressing the nucleic acid. ### Claim 8 (depends on 7) The nucleic acid of claim 7 in a host cell suitable for expressing the nucleic acid encoding the enolase.