# US 5,703,209 A · Amyloid precursor proteins and method of using same to assess agents which down-regulate formation of .beta.-amyloid peptide

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- Number: US 5,703,209 A
- Document ID: US5703209A
- Status: Granted
- Kind: A
- Application number: 08/464,248
- Filed: 1995-06-05
- Granted: 1997-12-30
- Earliest claimed: 1992-05-01
- Assignees: American Cyanamid Company
- Inventors: Michael Peter Vitek; Jack Steven Jacobsen
- Web page: https://patentgrep.com/patent/US5703209A

## CPC

- C07K 14/4711: {Alzheimer's disease; Amyloid plaque core protein}
- G01N 33/6896: {Neurological disorders, e.g. Alzheimer's disease}
- C07K 2319/00: Fusion polypeptide
- G01N 2333/4709: Amyloid plaque core protein
- G01N 2800/2821: Alzheimer
- Y10S 530/839: Nerves; brain
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

This application describes a purified and isolated fragment of a nucleic acid molecule encoding an amyloid precursor mutein, wherein the fragment comprises a nucleic acid sequence encoding at least one marker and a nucleic acid sequence of about 419, about 475 or about 494 amino acid residues in which a portion thereof encodes a .beta.-amyloid protein domain. Also described is a method for screening for a compound which reduces the formation of .beta.-amyloid protein.

## Claims

### Claim 1

A purified and isolated polypeptide encoded by the nucleic acid sequence of a nucleic acid molecule encoding an amyloid precursor mutein comprising a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform, wherein the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform.

### Claim 2

A purified and isolated polypeptide encoded by the nucleic acid sequence of a nucleic acid molecule encoding an amyloid precursor mutein comprising a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform, wherein the amino acid residues from position 11 to position 28 are deleted from the portion of the nucleic acid sequence encoding the .beta.-amyloid protein domain and the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform.

### Claim 3

A purified and isolated polypeptide encoded by the nucleic acid sequence of a nucleic acid molecule encoding an amyloid precursor mutein comprising a nucleic acid sequence encoding a marker and a nucleic acid sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform in which an alanine substitution at a phosphorylation site within the cytoplasmic domain of an amyloid precursor protein is encoded, wherein the nucleic acid molecule is an XbaI-SalI fragment of the gene encoding an amyloid precursor protein isoform.