# US 5,702,944 A · Microbial transport media

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- Number: US 5,702,944 A
- Document ID: US5702944A
- Status: Granted
- Kind: A
- Application number: 08/599,606
- Filed: 1996-02-09
- Granted: 1997-12-30
- Earliest claimed: 1994-07-25
- Assignees: Micro Test, Inc.
- Inventors: Stephen G. Racioppi; James P. Brinker
- Web page: https://patentgrep.com/patent/US5702944A

## CPC

- C12N 1/04: Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A microbial transport medium for the collection, transport and storage of samples suspected of having Chlamydia, Mycoplasma, Ureaplasma or viral pathogens comprises a balanced salt solution, a proteinaceous stabilizer, and carbohydrate and amino acid nutrient sources. The medium is buffered to maintain physiological pH and includes a pH indicator in order to indicate variation of pH outside the physiological pH range. The medium further comprises antimicrobial and antifungal agents and can include gelatin. Samples can be stored in the medium at temperatures ranging from room temperature to minus 70.degree. C. Additionally, the transport medium can be used in standardized commercial ELISA and PCR assays.

## Claims

### Claim 1

A aqueous transport medium capable of maintaining the viability of a plurality of microorganisms selected from the group consisting of Chlamydia, Mycoplasma, Ureaplasma, and viruses, said medium having physiological pH and comprising about 94-96.75 ml of water and:
______________________________________ gelatin (Bloom value 40-125) 0-20.0 g sugar 65-75 g HEPES 5-7 g KCl 0.3-0.6 g L-glutamic acid 0.5-1.0 g phenol red 5-15 mg CaCl.sub.2 0.1-0.5 g MgSO.sub.4.7H.sub.2 O 0.1-0.3 g bovine serum albumin 1.0-20.0 g vancomycin 0.01-0.05 g colistin 100,000-250,000 units amphotericin B 0.5-3.0 mg. ______________________________________

### Claim 2

An aqueous transport medium having physiological pH and comprising:
______________________________________ H.sub.2 O 24.89 liters sucrose 1711.43 g HEPES 148.99 g KCl 10.0 g L-glutamic acid 18.0 g phenol red 275 mg gelatin 125 g CaCl.sub.2 6.63 g MgSO.sub.4.7H.sub.2 O 5.0 g bovine serum albumin 125 g vancomycin 0.62 g colistin 5 .times. 10.sup.6 units amphotericin B 25.0 mg. ______________________________________

### Claim 3

An aqueous transport medium having physiological pH and comprising:
______________________________________ H.sub.2 O 24.89 liters sucrose 1711.43 g HEPES 148.99 g KCl 10.0 g L-glutamic acid 18.0 g phenol red 275 mg CaCl.sub.2 6.63 g MgSO.sub.4.7H.sub.2 O 5.0 g bovine serum albumin 250 g vancomycin 0.62 g colistin 5 .times. 10.sup.6 units amphotericin B 25.0 mg. ______________________________________

### Claim 4

An aqueous transport medium having physiological pH and comprising:
______________________________________ H.sub.2 O 24.89 liters sucrose 1711.43 g HEPES 148.99 g KCl 10.0 g L-glutamic acid 18.0 g phenol red 275 mg gelatin 125 g CaCl.sub.2 6.63 g MgSO.sub.4.7H.sub.2 O 5.0 g bovine serum albumin 125 g gentamicin 250 g amphotericin B 75.0 mg. ______________________________________